Highsmith A K, Abshire R L
Appl Microbiol. 1975 Oct;30(4):596-601. doi: 10.1128/am.30.4.596-601.1975.
A most-probable-number (MPN) technique was evaluated for detecting and enumerating Pseudomonas aeruginosa in water and wastewater. Both the presumptive and confirmatory media, as described in the 13th edition of Standard Methods for the Examination of Water and Wastewater, as well as modifications of these media were included in evaluations. Various samples of water were tested, namely chlorinated tap water, creek water, and influent to a wastewater treatment plant. Modified media repeatedly gave higher estimated MPNs of P. aeruginosa than media listed in Standard Methods. P. aeruginosa was detected and recovered from all creek water and wastewater samples, but not from tap water samples tested. This organism was determined to be present in as large numbers as the fecal coliforms and in even greater quantities than the fecal streptococci in all samples, whenever MPN estimations were determined from those positive tubes containing the modified confirmatory medium.
对一种最大可能数（MPN）技术进行了评估，以检测和计数水和废水中的铜绿假单胞菌。评估中包括了《水和废水检验标准方法》第13版中所述的推测性培养基和验证性培养基，以及这些培养基的改良型。对各种水样进行了测试，即加氯自来水、溪水和污水处理厂的进水。改良型培养基给出的铜绿假单胞菌估计MPN值反复高于《标准方法》中列出的培养基。在所有溪水和废水样本中都检测到并分离出了铜绿假单胞菌，但在所测试的自来水样本中未检测到。每当从含有改良型验证性培养基的阳性试管中确定MPN估计值时，在所有样本中，该菌的数量被确定与粪大肠菌群一样多，甚至比粪链球菌的数量更多。
