Yamamoto S, Shimomura Y, Kinoshita S, Nishida K, Yamamoto R, Tano Y
Department of Ophthalmology, Osaka University Medical School, Japan.
Am J Ophthalmol. 1994 Feb 15;117(2):160-3. doi: 10.1016/s0002-9394(14)73071-5.
We investigated the use of the polymerase chain reaction for detecting genomes of herpes simplex virus, varicella-zoster virus, and cytomegalovirus from tear film of patients with clinically diagnosed herpes simplex virus keratitis. Using the polymerase chain reaction with a herpes simplex virus detection sensitivity adjusted to 1.0 plaque-forming units/ml, we detected herpes simplex virus genomic sequences in 12 of 12 epithelial keratitis specimens, two of six stromal keratitis specimens, but in none of 20 normal specimens. Neither varicella-zoster virus nor cytomegalovirus genomic sequences were detected in any sample. These results suggest that polymerase chain reaction quickly performed with reduced sensitivity is useful as a diagnostic tool for confirming clinical observations.
我们研究了聚合酶链反应在检测临床诊断为单纯疱疹病毒性角膜炎患者泪膜中单纯疱疹病毒、水痘-带状疱疹病毒和巨细胞病毒基因组方面的应用。使用将单纯疱疹病毒检测灵敏度调整至1.0空斑形成单位/毫升的聚合酶链反应，我们在12份上皮性角膜炎标本中的12份、6份基质性角膜炎标本中的2份中检测到了单纯疱疹病毒基因组序列，但在20份正常标本中均未检测到。在任何样本中均未检测到水痘-带状疱疹病毒和巨细胞病毒基因组序列。这些结果表明，以降低的灵敏度快速进行的聚合酶链反应作为一种用于确认临床观察结果的诊断工具是有用的。
