Mohamed A M
J Oral Pathol. 1975 Oct-Nov;4(4):180-94. doi: 10.1111/j.1600-0714.1975.tb01741.x.
Human biopsy specimens from white patches on the mucosae of tongue and lips which had previously given positive cultures for Candida albicans were examined. The specimens were processed for electron microscopy by conventional techniques. The fine structure of the superficial layer of the epithelium revealed Candida albicans in extra- and intracellular sites. Penetration into the deeper layers was not observed. The fungus had a thick cell wall surrounded by an outer floccular layer. The cytoplasm of the superficial epithelial cells showed degenerative changes and fragmentation of tonofilaments in the immediate vicinity of the fungus. Microabscesses, formed by pools of neutrophils, were common in the upper spinous layer. In places, fungi and bacteria appear to have been phagocytosed by neutrophils and macrophages. The cells of the lower spinous and basal layers showed detachment of desmosomes, marked increase in intracellular glycogen, prominent lysosome-like dense bodies and abundant mitochondria of differing shapes. At the epithelia-connective tissue junction, fine filaments and collagen fibrils were intermingled throughout the basal lamina, forming multiple, highly electron-dense layers. A noticeable change in the connective tissue was the accumulation of numerous mast cells containing differently shaped granules filled with scrolls and dense strands of granular material. The terminal blood vessel exhibited leakage and numerous, concentric duplications of the basal lamina.
对取自舌部和唇部黏膜白色斑块的人体活检标本进行了检查，这些标本先前的白色念珠菌培养呈阳性。标本采用传统技术进行电子显微镜处理。上皮表层的精细结构显示白色念珠菌存在于细胞外和细胞内部位。未观察到其侵入更深层。真菌有一层厚细胞壁，周围有一层外部絮状层。在真菌紧邻区域，表层上皮细胞的细胞质显示出退行性变化以及张力丝断裂。由中性粒细胞聚集形成的微脓肿在上棘层很常见。在一些部位，真菌和细菌似乎已被中性粒细胞和巨噬细胞吞噬。棘层下层和基底层细胞显示桥粒分离、细胞内糖原显著增加、出现明显的溶酶体样致密小体以及大量不同形状的线粒体。在上皮 - 结缔组织交界处，细纤维和胶原纤维在整个基膜中相互交织，形成多个高电子密度层。结缔组织中一个明显变化是大量肥大细胞的聚集，这些肥大细胞含有形状各异的颗粒，颗粒内充满卷轴状和致密的颗粒物质束。终末血管出现渗漏，基膜有大量同心重复现象。
