Howlett J A, Squier C A
Infect Immun. 1980 Jul;29(1):252-60. doi: 10.1128/iai.29.1.252-260.1980.
The colonization and invasion of various animal oral mucosae by Candida albicans were examined in an organ culture model. Scanning and transmission electron microscopy of the oral epithelium between 12 and 30 h after inoculation with the fungus revealed the morphological relationships between host and parasite. Examination of the fungi in thin sections showed five distinct layers in the cell wall of C. albicans within the epithelium, but changes were evident in the organization and definition of the outer cell wall layers in budding hyphae and in hyphae participating in colonization and invasion of the epithelial cells. Adherence of the fungus to the superficial cells of the oral mucosa appeared to involve intimate contact between the epithelial cell surface and the deeper layers of the fungal cell wall. During invasion a close seal was maintained between the invading hyphae and the surrounding epithelial cell envelope, there being no other evidence of damage to the host cell surface except at the site of entry. Within the epithelial cells there was only occasional loss of cytoplasmic components in the vicinity of the invading hyphae. These findings would suggest that enzymatic lysis associated with the invasive process is localized and that the mechanical support provided by surface adherence and the intimate association between the fungus and the epithelial cell envelope may permit growth of Candida on through the epithelium.
在器官培养模型中研究了白色念珠菌对各种动物口腔黏膜的定植和侵袭情况。对接种真菌后12至30小时的口腔上皮进行扫描电镜和透射电镜观察，揭示了宿主与寄生虫之间的形态学关系。对薄切片中的真菌进行检查发现，上皮内白色念珠菌的细胞壁有五个不同的层，但在出芽菌丝以及参与上皮细胞定植和侵袭的菌丝中，外层细胞壁层的组织结构和清晰度有明显变化。真菌与口腔黏膜表层细胞的黏附似乎涉及上皮细胞表面与真菌细胞壁深层之间的紧密接触。在侵袭过程中，侵袭菌丝与周围上皮细胞包膜之间保持紧密密封，除了进入部位外，没有其他宿主细胞表面受损的证据。在上皮细胞内，侵袭菌丝附近偶尔会出现细胞质成分的丢失。这些发现表明，与侵袭过程相关的酶解作用是局部性的，表面黏附以及真菌与上皮细胞包膜之间的紧密结合所提供的机械支撑可能使念珠菌能够在上皮中持续生长。
