Chuma T, Yamada T, Yano K, Okamoto K, Yugi H
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 1994 Aug;56(4):697-700. doi: 10.1292/jvms.56.697.
A survey of Campylobacter jejuni in the cecal contents of broilers raised on a farm was carried out by the DNA-DNA hybridization method from the day of assignment to slaughter at about 1-week intervals. C. jejuni was detected in chickens as early as 1 week of age, and was widely detected at each week of age throughout the growing period. In addition, of 20 chickens tested just after assignment, 7 (35%) were C. jejuni positive. It is suggested that newly introduced chickens may have already been contaminated with C. jejuni. The hybridization method was able to detect C. jejuni in the chickens from the day of assignment to 3 weeks of age, but C. jejuni was never detected in the same chickens by the enriched culture method. Therefore, it is suggested that the hybridization method is more sensitive than the enriched culture method.
采用DNA-DNA杂交法，对某农场饲养的肉鸡从分配之日起至屠宰前每隔约1周对盲肠内容物中的空肠弯曲菌进行一次检测。空肠弯曲菌在鸡1周龄时就已被检测到，并且在整个生长周期的各周龄时均广泛被检测到。此外，在分配后刚检测的20只鸡中，有7只（35%）为空肠弯曲菌阳性。这表明新引入的鸡可能已经被空肠弯曲菌污染。杂交法能够在分配之日起至3周龄的鸡中检测到空肠弯曲菌，但富集培养法在相同的鸡中从未检测到空肠弯曲菌。因此，提示杂交法比富集培养法更敏感。
