Hirai K, Yamada M, Arao Y, Kato S, Nii S
Department of Cell Regulation, Tokyo Medical and Dental University, Japan.
Arch Virol. 1990;114(3-4):153-65. doi: 10.1007/BF01310745.
We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cells. Examination of the infectious virus by field inversion gel electrophoresis revealed the presence of DNA of about 180 kbp in both the culture fluid and cell fractions of the infected MSB-41C cells. The DNA was found to consist of Marek's disease virus (MDV) serotype 2 (MDV2) and MDV serotype 1 (MDV1) DNA by Southern blot hybridization. The MDV1 DNA consisted of sequences mainly from the long inverted repeats including multiple copies of 132 bp direct tandem repeats. Molecular cloning of BamHI digests of the MDV2 DNA revealed a fragment of MDV1 DNA and MDV2 DNA fused together, indicating that the recombinant MDV2 DNA had been generated by genetic recombination with the latent MDV1 DNA.
我们对在马立克氏病淋巴母细胞系MSB1-41C中生长良好、能诱导形成以多核巨细胞为特征的细胞病变效应的疱疹型病毒特性进行了表征。通过场反转凝胶电泳对感染性病毒进行检测，发现在感染的MSB-41C细胞的培养液和细胞组分中均存在约180 kbp的DNA。通过Southern印迹杂交发现该DNA由马立克氏病病毒2型（MDV2）和马立克氏病病毒1型（MDV1）DNA组成。MDV1 DNA由主要来自长反向重复序列的序列组成，包括多个132 bp直接串联重复序列的拷贝。对MDV2 DNA的BamHI酶切片段进行分子克隆，发现一个MDV1 DNA片段和MDV2 DNA片段融合在一起，表明重组MDV2 DNA是通过与潜伏的MDV1 DNA发生基因重组产生的。
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