马立克氏病病毒2型（MDV）的限制性内切酶图谱及MDV三种血清型之间的共线性关系。
The restriction endonuclease map of Marek's disease virus (MDV) serotype 2 and collinear relationship among three serotypes of MDV.
作者信息
Ono M, Katsuragi-Iwanaga R, Kitazawa T, Kamiya N, Horimoto T, Niikura M, Kai C, Hirai K, Mikami T
机构信息
Department of Veterinary Microbiology, University of Tokyo.
出版信息
Virology. 1992 Nov;191(1):459-63. doi: 10.1016/0042-6822(92)90210-g.
A BamHI, EcoRI, and XhoI restriction endonuclease map of Marek's disease virus (MDV) serotype 2 (MDV2) DNA was constructed by double-digest analyses of 28 cloned BamHI and 11 cloned EcoRI fragments of MDV2 DNA, followed by hybridization tests of these cloned BamHI DNA fragments with electrophoretically separated digests of MDV2-infected cell DNA. On this map, MDV2 genome consisted of two segments which have unique regions inserted between two inverted repeat regions as observed in MDV serotype 1 and 3 genomes. Further, the DNA homology among three serotypes of MDV was examined by hybridization under less stringent conditions using cloned BamHI fragments of MDV2 DNA. Most of the MDV2 fragments located within the unique regions hybridized with MDV serotype 1 and 3 DNAs, indicating the presence of the collinear relationship among three serotypes. In addition, MDV2 DNA fragments which hybridized with the DNA fragments encoding MDV1 gp57-65 (or A antigen) or MDV1 gp100, gp60, gp49 (or B antigen) were identified and these fragments of serotypes 1 and 2 found to be collinear.
通过对28个克隆的马立克氏病病毒2型（MDV2）DNA的BamHI片段和11个克隆的EcoRI片段进行双酶切分析，并随后用这些克隆的BamHI DNA片段与MDV2感染细胞DNA的电泳分离酶切产物进行杂交试验，构建了MDV2 DNA的BamHI、EcoRI和XhoI限制性内切酶图谱。在该图谱上，MDV2基因组由两个片段组成，这两个片段在两个反向重复区域之间插入了独特区域，正如在MDV1型和3型基因组中所观察到的那样。此外，使用MDV2 DNA的克隆BamHI片段，在较低严谨条件下通过杂交检测了MDV三种血清型之间的DNA同源性。位于独特区域内的大多数MDV2片段与MDV1型和3型DNA杂交，表明三种血清型之间存在共线性关系。此外，还鉴定出了与编码MDV1 gp57 - 65（或A抗原）或MDV1 gp100、gp60、gp49（或B抗原）的DNA片段杂交的MDV2 DNA片段，并且发现1型和2型的这些片段是共线的。
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