Jang H K, Ono M, Kato Y, Tohya Y, Niikura M, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Arch Virol. 1996;141(11):2207-16. doi: 10.1007/BF01718226.
The gene of Marek's disease virus (MDV) serotype 2 (MDV2) homologous to glycoprotein D (gD) of herpes simplex virus (HSV) was identified and characterized by its nucleotide and predicted amino acid sequences. The MDV2 gD homologous gene contains an open reading frame capable of specifying a polypeptide of 385 amino acids, which include N- and C-terminal hydrophobic domains consistent with signal and anchor regions, respectively, and two potential N-linked glycosylation sites, one of which was located in a highly conserved region when compared to MDV serotype 1 (MDV1) and herpesvirus of turkeys (HVT). By northern blot analysis using a MDV2 gD-specific DNA probe, two highly abundant polycistronic 6.0 and 4.2 kb transcripts were detected in MDV2-infected cells. The genes encoding MDV2 protein kinase (PK), gD, and glycoprotein I (gI) homologues are transcribed to form 3' coterminal mRNAs of 6.0 kb (encoding PK, gD and gI) and 4.2 kb (encoding gD and gI), respectively. By using rapid amplification cDNA end (RACE) method, several RNA start sites, to be thought those of the 4.2 kb mRNA, were detected in the upstream of MDV2 gD homologue.
通过其核苷酸和预测的氨基酸序列，鉴定并表征了与单纯疱疹病毒（HSV）糖蛋白D（gD）同源的马立克氏病病毒2型（MDV2）基因。MDV2 gD同源基因包含一个开放阅读框，能够编码一个由385个氨基酸组成的多肽，该多肽分别包含与信号区和锚定区一致的N端和C端疏水结构域，以及两个潜在的N-糖基化位点，其中一个与1型马立克氏病病毒（MDV1）和火鸡疱疹病毒（HVT）相比，位于一个高度保守的区域。使用MDV2 gD特异性DNA探针进行Northern印迹分析，在MDV2感染的细胞中检测到两种高度丰富的多顺反子6.0和4.2 kb转录本。编码MDV2蛋白激酶（PK）、gD和糖蛋白I（gI）同源物的基因分别转录形成6.0 kb（编码PK、gD和gI）和4.2 kb（编码gD和gI）的3'共末端mRNA。通过使用快速扩增cDNA末端（RACE）方法，在MDV2 gD同源物上游检测到几个RNA起始位点，被认为是4.2 kb mRNA的起始位点。
