通过单管巢式聚合酶链反应对沙眼衣原体眼部菌株进行基因分型。
Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.
作者信息
Pecharatana S, Pickett M A, Watt P J, Ward M E
机构信息
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, UK.
出版信息
PCR Methods Appl. 1993 Dec;3(3):200-4. doi: 10.1101/gr.3.3.200.
A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.
本文描述了一种单管巢式聚合酶链反应（PCR），它能够区分眼部感染沙眼衣原体A、B或C血清型。该方法使用用不同荧光染料标记的基因型特异性引物、“插入/缺失”PCR扩增，并使用GENESCAN 672软件在应用生物系统公司373A DNA测序仪上进行产物分析。该系统能够轻松检测同一只眼睛内A和B血清型的混合感染。这种策略提供了一种适用于广泛流行病学研究的快速基因分型方法。
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