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1
Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.
2
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.人生长激素：通过激素两个片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
3
Human somatotropin: biological characterization of the recombinant molecule.人生长激素：重组分子的生物学特性
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5. doi: 10.1073/pnas.73.10.3463.
4
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
5
Two contiguous thrombin fragments of human somatotropin form a functionally active recombinant, but the two homologous fragments from sheep hormone do not.人生长激素的两个相邻凝血酶片段形成了一种功能活跃的重组体，但来自绵羊激素的两个同源片段却不能。
Biochemistry. 1981 Dec 8;20(25):7251-8. doi: 10.1021/bi00528a031.
6
Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.
7
Semisynthesis of human somatotropin analogs.人生长激素类似物的半合成
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.
8
Human somatotropin: semisynthesis of the hormone by noncovalent interaction of the NH2-terminal fragment with synthetic analogs of the COOH-terminal fragment.人生长激素：通过氨基末端片段与羧基末端片段的合成类似物的非共价相互作用对该激素进行半合成。
Biochem Biophys Res Commun. 1978 May 15;82(1):217-22. doi: 10.1016/0006-291x(78)90598-3.
9
Dissociation kinetics of tetra-S-carbamidomethylated plasmin-modified human somatotropin.四-S-氨甲酰甲基化纤溶酶修饰的人生长激素的解离动力学
Biochemistry. 1978 Aug 8;17(16):3315-20. doi: 10.1021/bi00609a022.
10
Recombinant hormones from fragments of human growth hormone and human placental lactogen.来自人生长激素和人胎盘催乳素片段的重组激素。
J Biol Chem. 1981 Jan 10;256(1):296-300.

引用本文的文献

1
Human growth hormone: 1974-1981.人生长激素：1974 - 1981年。
Mol Cell Biochem. 1982 Jul 7;46(1):31-41. doi: 10.1007/BF00215579.
2
Human somatotropin and lipotropin: relation between structure and activity.人生长激素和促脂解素：结构与活性之间的关系。
Can Med Assoc J. 1979 Mar 3;120(5):575-8.
3
Semisynthesis of human somatotropin analogs.人生长激素类似物的半合成
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.
4
The disulfide bond connecting the chains of ricin.连接蓖麻毒素各链的二硫键。
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1096-100. doi: 10.1073/pnas.75.3.1096.
5
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
6
Preparation of a disulfide-linked dimer of human placental lactogen fragment 1--134 with immunologic and biologic activity.具有免疫和生物活性的人胎盘催乳素片段1 - 134的二硫键连接二聚体的制备
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1204-7. doi: 10.1073/pnas.76.3.1204.
7
Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.绵羊催乳素纤维蛋白溶酶消化产生的两个片段：特性鉴定及重组以产生完全免疫反应性
Proc Natl Acad Sci U S A. 1978 May;75(5):2155-9. doi: 10.1073/pnas.75.5.2155.

本文引用的文献

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Bioassay of hypophyseal growth hormone; the tibia test.垂体生长激素的生物测定；胫骨试验。
Endocrinology. 1949 Nov;45(5):455-63, illust. doi: 10.1210/endo-45-5-455.
2
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
3
Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.人垂体生长激素。VI. 改进的分离方法及氨基末端氨基酸序列
Arch Biochem Biophys. 1962 Sep;Suppl 1:327-32.
4
Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants.用放射免疫分析法测定大鼠血浆生长激素浓度：性别、妊娠、哺乳、麻醉、垂体切除及垂体鞍外移植的影响。
Endocrinology. 1966 Aug;79(2):275-80. doi: 10.1210/endo-79-2-275.
5
Human pituitary growth hormone. Physicochemical investigations of the native and reduced-alkylated protein.人生长激素。天然及还原烷基化蛋白质的物理化学研究。
Biochemistry. 1969 Dec;8(12):4701-8. doi: 10.1021/bi00840a007.
6
Comparative studies of the single tryptophan residue in human chorionic somatomammotropin and human pituitary growth hormone.人绒毛膜生长催乳素和人垂体生长激素中单个色氨酸残基的比较研究。
Biochemistry. 1972 Oct 24;11(22):4179-87. doi: 10.1021/bi00772a023.
7
Human pituitary growth hormone: immunochemical investigations of biologically active fragments.人垂体生长激素：生物活性片段的免疫化学研究
Arch Biochem Biophys. 1974 Oct;164(2):571-4. doi: 10.1016/0003-9861(74)90068-x.
8
Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.人垂体生长激素：纤溶酶消化产物中两个生物活性片段的分离与特性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201. doi: 10.1073/pnas.71.4.1197.
9
The synthesis and biological activity of (165, 182, 189-S-carbamidomethylcysteine)-human growth hormone-(140-191).（165, 182, 189-S-氨甲酰甲基半胱氨酸）-人生长激素-（140-191）的合成与生物活性
Int J Pept Protein Res. 1975;7(6):495-501. doi: 10.1111/j.1399-3011.1975.tb02471.x.
10
Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.

人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。

Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.

作者信息

Li C H, Bewley T A, Blake J, Hayashida T

出版信息

Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.

DOI:10.1073/pnas.74.3.1016
PMID:265545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430568/
Abstract

The recombinant hormone obtained by noncovalent interaction of the natural NH2-terminal fragment (consisting of 134 amino acid residues) with a synthetic COOH-terminal fragment of 52 amino acids of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit full biological activity of the native hormone as evidenced by the tibia test. Radioimmunoassay data indicate that the semisynthetic recombinant hormone possesses essentially full immunoreactivity as compared to the native one. In addition, circular dichroism and fluorescence emission spectra of the semisynthetic recombinant are identical to that of the undisociated, plasmin modified, reduced-carbamidomethylated hormone.

摘要

通过天然NH2末端片段（由134个氨基酸残基组成）与还原氨甲酰甲基化人生长激素分子的52个氨基酸的合成COOH末端片段的非共价相互作用获得的重组激素，经胫骨试验证明具有天然激素的完全生物活性。放射免疫测定数据表明，与天然激素相比，半合成重组激素具有基本完全的免疫反应性。此外，半合成重组体的圆二色性和荧光发射光谱与未解离的、纤溶酶修饰的、还原氨甲酰甲基化激素的光谱相同。