• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人生长激素类似物的半合成

Semisynthesis of human somatotropin analogs.

作者信息

Li C H, Blake J

出版信息

Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.

DOI:10.1073/pnas.76.12.6124
PMID:293709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411815/
Abstract

Complementation of the natural NH2-terminal 134-amino acid fragment of the reduced, carbamoylmethylated human somatotropin with synthetic analogs of COOH-terminal fragments of 57, 42, or 38 amino acids of reduced, carbamoyl-methylated human somatotropin has been investigated. It was found that synthetic fragments of 57 and 42 amino acids gave recombinants with full growth-promoting activity, whereas attempts to obtain a recombinant with the synthetic 38-amino acid fragment were unsuccessful. The synthesis of two analogs of the COOH-terminal fragment of human somatotropin, human [N]e170, Ala165,182,189] somatotropin-(150-191) and human [Nle170, Ala165,182,189] somatotropin-(154-191), is herein described.

摘要

已对还原的、氨甲酰甲基化的人生长激素天然氨基末端134个氨基酸片段与还原的、氨甲酰甲基化的人生长激素57、42或38个氨基酸的羧基末端片段的合成类似物进行互补研究。发现57个和42个氨基酸的合成片段产生具有完全促生长活性的重组体，而用38个氨基酸的合成片段获得重组体的尝试未成功。本文描述了人生长激素羧基末端片段的两种类似物，即人[N]e170，Ala165,182,189]生长激素-(150 - 191)和人[Nle170，Ala165,182,189]生长激素-(154 - 191)的合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efef/411815/4e147f8d03b6/pnas00012-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efef/411815/4e147f8d03b6/pnas00012-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efef/411815/4e147f8d03b6/pnas00012-0110-a.jpg

相似文献

1
Semisynthesis of human somatotropin analogs.人生长激素类似物的半合成
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.
2
Human somatotropin. 55. Synthesis and growth-promoting activity of peptide analogs of the carboxyl terminal plasmin fragment.人生长激素。55. 羧基末端纤溶酶片段肽类似物的合成及促生长活性。
Int J Pept Protein Res. 1978 May;11(5):315-22.
3
Human somatotropin: noncovalent interactions of the natural NH2-terminal 1-134 segment with synthetic analogs of the COOH-terminal fragment.人生长激素：天然氨基末端1 - 134片段与羧基末端片段合成类似物的非共价相互作用。
Arch Biochem Biophys. 1985 May 15;239(1):12-7. doi: 10.1016/0003-9861(85)90806-9.
4
Human somatotropin: semisynthesis of the hormone by noncovalent interaction of the NH2-terminal fragment with synthetic analogs of the COOH-terminal fragment.人生长激素：通过氨基末端片段与羧基末端片段的合成类似物的非共价相互作用对该激素进行半合成。
Biochem Biophys Res Commun. 1978 May 15;82(1):217-22. doi: 10.1016/0006-291x(78)90598-3.
5
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
6
Human somatotropin: semisynthesis of the hormone by noncovalent interaction of the natural NH2-terminal fragment with a synthetic analog of the COOH-terminal fragment.人生长激素：通过天然氨基末端片段与羧基末端片段的合成类似物的非共价相互作用对该激素进行半合成。
Arch Biochem Biophys. 1981 Oct 1;211(1):338-45. doi: 10.1016/0003-9861(81)90462-8.
7
Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.
8
Human somatotropin: biological characterization of the recombinant molecule.人生长激素：重组分子的生物学特性
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5. doi: 10.1073/pnas.73.10.3463.
9
Immunoreactivity and receptor binding of mixed recombinants of human growth hormone and chorionic somatomammotropin.人生长激素与绒毛膜促生长催乳素混合重组体的免疫反应性及受体结合
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5391-4. doi: 10.1073/pnas.75.11.5391.
10
Human growth hormone: 1974-1981.人生长激素：1974 - 1981年。
Mol Cell Biochem. 1982 Jul 7;46(1):31-41. doi: 10.1007/BF00215579.

引用本文的文献

1
Human growth hormone: 1974-1981.人生长激素：1974 - 1981年。
Mol Cell Biochem. 1982 Jul 7;46(1):31-41. doi: 10.1007/BF00215579.
2
Human somatotropin: covalent reconstitution of two polypeptide contiguous fragments with thrombin.人生长激素：用凝血酶对两个多肽连续片段进行共价重建。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6135-8. doi: 10.1073/pnas.78.10.6135.

本文引用的文献

1
Bioassay of hypophyseal growth hormone; the tibia test.垂体生长激素的生物测定；胫骨试验。
Endocrinology. 1949 Nov;45(5):455-63, illust. doi: 10.1210/endo-45-5-455.
2
SOLID-PHASE PEPTIDE SYNTHESIS. 3. AN IMPROVED SYNTHESIS OF BRADYKININ.固相肽合成。3. 缓激肽的改进合成法。
Biochemistry. 1964 Sep;3:1385-90. doi: 10.1021/bi00897a032.
3
Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.人垂体生长激素。VI. 改进的分离方法及氨基末端氨基酸序列
Arch Biochem Biophys. 1962 Sep;Suppl 1:327-32.
4
[A new method for synthesis of peptides: activation of the carboxyl group with dicyclohexylcarbodiimide using 1-hydroxybenzotriazoles as additives].[一种合成肽的新方法：以1-羟基苯并三唑为添加剂，用二环己基碳二亚胺活化羧基]
Chem Ber. 1970;103(3):788-98. doi: 10.1002/cber.19701030319.
5
Increased coupling yields in solid phase peptide synthesis with a modified carbodiimide coupling procedure.采用改良的碳二亚胺偶联方法提高固相肽合成中的偶联产率。
Hoppe Seylers Z Physiol Chem. 1972 Dec;353(12):1973-6.
6
Adrenocorticotropins. 44. Total synthesis of the human hormone by the solid-phase method.促肾上腺皮质激素。44. 用固相法全合成人体激素。
J Am Chem Soc. 1973 Feb 21;95(4):1310-5. doi: 10.1021/ja00785a049.
7
Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.人垂体生长激素：纤溶酶消化产物中两个生物活性片段的分离与特性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201. doi: 10.1073/pnas.71.4.1197.
8
The synthesis and biological activity of (165, 182, 189-S-carbamidomethylcysteine)-human growth hormone-(140-191).（165, 182, 189-S-氨甲酰甲基半胱氨酸）-人生长激素-（140-191）的合成与生物活性
Int J Pept Protein Res. 1975;7(6):495-501. doi: 10.1111/j.1399-3011.1975.tb02471.x.
9
Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.
10
Human somatotropin. 55. Synthesis and growth-promoting activity of peptide analogs of the carboxyl terminal plasmin fragment.人生长激素。55. 羧基末端纤溶酶片段肽类似物的合成及促生长活性。
Int J Pept Protein Res. 1978 May;11(5):315-22.