Hjelm H
Scand J Immunol. 1975;4(7):633-40. doi: 10.1111/j.1365-3083.1975.tb02671.x.
Affinity chromatography of IgG on protein A-Sepharose was used to isolate the human subclass IgG3 from normal serum and from a patient with multiple myeloma. The isolated material was purified by chromatography on Sephadex G-150 and characterized immunochemically. Ultracentrifugation studies gave so20,w values of about 6.80 for both normal and myeloma IgG3. Approximately 54 half-cystine residues per molecule of IgG3 were obtained as judged from amino acid analysis after performic acid oxidation of the proteins. Polyacrylamide gel electrophoresis in 0.1% sodium dodecyl sulfate of the isolated and reduced material resulted in two bands corresponding to molecular weights of approximately 23,000 and 56,000, respectively. The yield of normal human IgG3 represented 1%-2% of the total IgG.
利用IgG在蛋白A-琼脂糖凝胶上的亲和层析，从正常血清和一名多发性骨髓瘤患者的血清中分离出人IgG3亚类。分离得到的物质经Sephadex G-150柱层析纯化，并进行免疫化学鉴定。超速离心研究表明，正常和骨髓瘤来源的IgG3的沉降系数（s20,w）约为6.80。经过甲酸氧化蛋白质后进行氨基酸分析判断，每分子IgG3约有54个半胱氨酸残基。分离并还原后的物质在0.1%十二烷基硫酸钠中进行聚丙烯酰胺凝胶电泳，得到两条带，分别对应分子量约为23,000和56,000。正常人IgG3的产量占总IgG的1%-2%。
