Vandamme P, Segers P, Vancanneyt M, van Hove K, Mutters R, Hommez J, Dewhirst F, Paster B, Kersters K, Falsen E
Laboratorium voor Microbiologie, Faculteit Wetenschappen, University of Ghent, Belgium.
Int J Syst Bacteriol. 1994 Jan;44(1):24-37. doi: 10.1099/00207713-44-1-24.
The phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. These strains constitute a genotypically homogeneous taxon in rRNA superfamily V, as shown by DNA-rRNA hybridization data. Determination of the 16S rRNA sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by Gherna and Woese (R. Gherna and C. R. Woese, Syst. Appl. Microbiol. 15:513-521, 1992). This new taxon is only distantly related to other members of the "flavobacter-bacteroides" phylum and is therefore given separate generic status. The DNA-DNA binding values for members of this taxon, for which we propose the name Ornithobacterium rhinotracheale, confirmed that all of the strains are highly interrelated (DNA-DNA binding values greater than 90% were measured). The G+C contents of members of this taxon are between 37 and 39 mol%. An analysis of the cellular proteins and fatty acids and classical phenotypic characteristics allowed us to distinguish O. rhinotracheale from phenotypically similar taxa, such as Riemerella anatipestifer and Capnocytophaga species. The respiratory quinone content (menaquinone 7) and carbohydrate pattern of O. rhinotracheale conform with the respiratory quinone contents and carbohydrate patterns of other members of rRNA superfamily V.
对21株革兰氏阴性禽类分离株的系统发育位置以及各种基因型、化学分类学和经典表型特征进行了研究。如DNA-rRNA杂交数据所示，这些菌株在rRNA超家族V中构成一个基因型均一的分类单元。对该分类单元的16S rRNA序列进行测定，揭示了其在Gherna和Woese（R. Gherna和C. R. Woese，《系统应用微生物学》15:513 - 521，1992年）所描述的“黄杆菌 - 拟杆菌”门的“黄杆菌”亚组中的详细位置。这个新的分类单元与“黄杆菌 - 拟杆菌”门的其他成员仅具有远缘关系，因此赋予其独立的属地位。我们提议将该分类单元的成员命名为气管鸟杆菌（Ornithobacterium rhinotracheale），其DNA - DNA结合值证实所有菌株高度相关（测得的DNA - DNA结合值大于90%）。该分类单元成员的G + C含量在37至39摩尔%之间。对细胞蛋白质、脂肪酸和经典表型特征的分析使我们能够将气管鸟杆菌与表型相似的分类单元区分开来，如鸭疫里默氏菌（Riemerella anatipestifer）和二氧化碳嗜纤维菌属（Capnocytophaga）的物种。气管鸟杆菌的呼吸醌含量（甲基萘醌7）和碳水化合物模式与rRNA超家族V的其他成员的呼吸醌含量和碳水化合物模式一致。
