Titley O G, Cooper M, Thomas A, Hancock K
West Midlands Regional Plastic and Jaw Surgery Unit, Wordsley Hospital.
Br J Plast Surg. 1994 Jan;47(1):24-9. doi: 10.1016/0007-1226(94)90113-9.
Quantitative bacteriology is presented on 102 consecutive split-skin grafts. A sample of graft was cultured whenever skin was taken and whenever stored skin was used. Stored skin unused at 21 days was also cultured. The percentage take of grafts was inversely proportional to number of organisms/g of skin (r = -0.24, p < 0.05). Commonly used storage conditions facilitated bacterial multiplication. There was no significant difference in organisms/g contaminating grafts for different surgeons, skin preparations, types of grafts, cutting tools or mode of anaesthesia. Male patients had a significantly greater count for initial grafts (p = 0.02), but after 3 weeks storage there was no sex difference.
对连续102例分层皮片移植进行了定量细菌学研究。每次取皮时以及每次使用储存皮片时，均对移植皮片样本进行培养。对21天时未使用的储存皮片也进行了培养。移植皮片的成活率与每克皮肤中的微生物数量呈负相关（r = -0.24，p < 0.05）。常用的储存条件促进了细菌繁殖。不同外科医生、皮肤准备方法、移植皮片类型、切割工具或麻醉方式对每克污染移植皮片的微生物数量没有显著差异。男性患者初始移植皮片的细菌计数显著更高（p = 0.02），但储存3周后不存在性别差异。
