Kubo K, Kakimoto T, Kanda C, Tsukasa N, Uehara M, Izumi Y, Kamada T, Kaneko N, Sueda T
Department of Periodontology, Kagoshima University Dental School, Japan.
J Biomed Mater Res. 1993 Sep;27(9):1175-80. doi: 10.1002/jbm.820270908.
The effects of bioactive glass and vitamin D3 on cultured fibroblasts derived from periodontal-ligament, with respect to their proliferation and alkaline-phosphatase activity were studied. The cells were cultured with or without the bioactive glass and/or vitamin D3, then the number and alkaline-phosphatase activity of the cells were measured periodically until the 33rd day. Formation of mineralized deposits was assessed by staining with alizarin red and von Kossa staining techniques. Fewer fibroblasts grew when they were cultured in the presence of bioactive glass and/or vitamin D3 as compared to those cultures without them. Alkaline-phosphatase activity was greater in the fibroblasts cultured with bioactive glass and vitamin D3 than in the cells grown without them. Mineralized deposits assessed by alizarin red and von Kossa staining techniques were observed microscopically around the fibroblasts cultured with bioactive glass and/or vitamin D3. A nodule visible after drying was evident only when both bioactive glass and vitamin D3 were present in culture. The results showed that although the bioactive glass and vitamin D3 decreased cell proliferation, they increased the alkaline-phosphatase activity of the fibroblasts which formed a nodule, suggesting an effect which might be useful for implant materials.
研究了生物活性玻璃和维生素D3对源自牙周韧带的培养成纤维细胞增殖和碱性磷酸酶活性的影响。细胞在有或无生物活性玻璃和/或维生素D3的情况下进行培养，然后定期测量细胞数量和碱性磷酸酶活性，直至第33天。通过茜素红染色和冯·科萨染色技术评估矿化沉积物的形成。与未添加生物活性玻璃和/或维生素D3的培养物相比，在添加它们的情况下培养的成纤维细胞生长较少。与未添加生物活性玻璃和维生素D3的细胞相比，用生物活性玻璃和维生素D3培养的成纤维细胞碱性磷酸酶活性更高。通过茜素红和冯·科萨染色技术评估，在添加生物活性玻璃和/或维生素D3培养的成纤维细胞周围显微镜下观察到矿化沉积物。仅当培养物中同时存在生物活性玻璃和维生素D3时，干燥后可见的结节才明显。结果表明，尽管生物活性玻璃和维生素D3降低了细胞增殖，但它们增加了形成结节的成纤维细胞的碱性磷酸酶活性，提示这一效应可能对植入材料有用。
