Division of Biomaterials and Bioengineering, University of California, San Francisco, California 94143-0758, USA.
J Biomed Mater Res A. 2011 Aug;98(2):177-84. doi: 10.1002/jbm.a.33102. Epub 2011 May 4.
This study resulted in enhanced collagen type 1 and osteocalcin expression in human periodontal ligament fibroblasts (hPDLF) when exposed to bioactive glass conditioned media that subsequently may promote early mineralized tissue development. Commercial Bioglass™ (45S5) and experimental bioactive coating glass (6P53-b), were used to make a glass conditioned media (GCM) for comparison to control medium. ICP-MS analysis showed increased concentrations of Ca(2+), PO(4) (3-), Si(4+), and Na(+), for 45S5 GCM and Mg(2+), K(+), Ca(2+), PO(4)(3-), Si(4+), and Na(+) for 6P53-b GCM (relative to control medium). Differentiating hPDLF cultures exposed to 45S5 and 6P53-b GCM showed enhanced expression of collagen type 1 (Col1α1, Col1α2), osteocalcin, and alkaline phosphatase gene expression. These GCM also enhanced osteocalcin protein expression. After 16 d of culture, 45S5 and 6P53-b GCM treated cells showed regions of deep red Alizarin staining, indicating increased Ca within their respective extracellular matrices (ECM), while control-treated cells did not exhibit these features. SEM analysis showed more developed ECM in GCM treated cultures, indicated by multiple tissue layering and abundant collagen fiber bundle formation, while control treated cells did not exhibit these features. SEM analysis showed polygonal structures suggestive of CaP in 45S5 GCM treated cultures. These results indicate the osteogenic potential of bioactive coating glass in periodontal bone defect filling applications.
这项研究表明，当人牙周韧带成纤维细胞（hPDLF）暴露于生物活性玻璃条件培养基中时，胶原蛋白 1 型和骨钙素的表达增强，这可能促进早期矿化组织的发育。使用商业 Bioglass™（45S5）和实验性生物活性涂层玻璃（6P53-b）来制备玻璃条件培养基（GCM），以与对照培养基进行比较。ICP-MS 分析显示，45S5 GCM 中 Ca(2+)、PO(4)(3-)、Si(4+)和 Na(+)的浓度增加，6P53-b GCM 中 Mg(2+)、K(+)、Ca(2+)、PO(4)(3-)、Si(4+)和 Na(+)的浓度增加（相对于对照培养基）。暴露于 45S5 和 6P53-b GCM 的分化 hPDLF 培养物显示胶原蛋白 1 型（Col1α1、Col1α2）、骨钙素和碱性磷酸酶基因表达增强。这些 GCM 还增强了骨钙素蛋白的表达。培养 16 天后，45S5 和 6P53-b GCM 处理的细胞显示出深红色茜素染色区域，表明其细胞外基质（ECM）中 Ca 增加，而对照处理的细胞没有表现出这些特征。SEM 分析显示，GCM 处理的培养物中 ECM 更发达，表现为多层组织和丰富的胶原纤维束形成，而对照处理的细胞没有表现出这些特征。SEM 分析显示，45S5 GCM 处理的培养物中存在多边形结构，提示存在 CaP。这些结果表明生物活性涂层玻璃在牙周骨缺损填充应用中的成骨潜力。
