Kubo K, Tsukasa N, Uehara M, Izumi Y, Ogino M, Kitano M, Sueda T
Department of Periodontology, Kagoshima University Dental School, Japan.
J Oral Rehabil. 1997 Jan;24(1):70-5. doi: 10.1046/j.1365-2842.1997.00462.x.
Previously, the authors reported that bioactive glass promoted formation of nodules in cultured periodontal-ligament fibroblasts and high concentrations of calcium and silicon were detected in the culture medium. Herein, the effects of bioactive glass, calcium alone and calcium and silicon for formation of nodules on the periodontal-ligament fibroblasts are examined. The cells were cultured with bioactive glass, without bioactive glass, calcium and silicon, with calcium alone and with calcium and silicon. The cell growth, alkaline-phosphatase activity, amount of pro-collagen type I and the number of nodules were measured periodically until the 21st day. The cell growth rate with calcium and silicon was the highest, however the rate with bioactive glass and calcium alone were similar to that without them. The alkaline-phosphatase activity and the amount of pro-collagen showed peaks at the 11th day. At the peak, the alkaline-phosphatase activity with bioactive glass or calcium and silicon, and the pro-collagen with bioactive glass was higher than without them. The number of nodules with bioactive glass, calcium alone or calcium and silicon was greater than without them. These findings suggested that the combination of calcium and silicon released from bioactive glass was concerned with the formation of nodules on the periodontal-ligament cells.
此前，作者报道生物活性玻璃可促进培养的牙周膜成纤维细胞形成结节，且在培养基中检测到高浓度的钙和硅。在此，研究生物活性玻璃、单独的钙以及钙和硅对牙周膜成纤维细胞形成结节的影响。将细胞分别与生物活性玻璃、不添加生物活性玻璃、钙和硅、单独的钙以及钙和硅一起培养。定期测量细胞生长、碱性磷酸酶活性、I型前胶原含量以及结节数量，直至第21天。钙和硅组的细胞生长速率最高，但生物活性玻璃组和单独钙组的速率与不添加它们的组相似。碱性磷酸酶活性和I型前胶原含量在第11天达到峰值。在峰值时，生物活性玻璃组或钙和硅组的碱性磷酸酶活性以及生物活性玻璃组的I型前胶原含量均高于不添加它们的组。生物活性玻璃组、单独钙组或钙和硅组的结节数量均多于不添加它们的组。这些发现表明，生物活性玻璃释放的钙和硅的组合与牙周膜细胞上结节的形成有关。
