Shanbhag A S, Jacobs J J, Black J, Galante J O, Glant T T
Department of Orthopedic Surgery, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
J Biomed Mater Res. 1994 Jan;28(1):81-90. doi: 10.1002/jbm.820280111.
Particulate wear-debris are detected in histiocytes/macrophages of granulomatous tissues adjacent to loose joint prostheses. Such cell-particle interactions have been simulated in vitro by challenging macrophages with particles dosed according to weight percent, volume percent, and number of particles. Each of these dosage methods has inherent shortcomings due to varying size and density of challenging particles of different compositions. In this study we challenged P388D1 macrophages with titania and polystyrene particles (< 2 microns), with dosage based on the ratio of the surface area of the particles to the surface area of the cells. The effect of size and composition on (1) the bone resorbing activity, (2) fibroblast proliferation, and (3) secretion of IL-1 and PGE2 was determined. Macrophage response to particulate debris appears to be dependent on particle size, composition, and dose as given by surface area ratio. P388D1 macrophages challenged with titania particles released IL-1, but did not stimulate fibroblasts. Inhibition of macrophage DNA synthesis at higher surface area ratios suggests cell damage or death. Particle-stimulated cells increased bone resorption up to 125% of controls but released only basal levels of PGE2. Macrophages stimulated by wear particles are expected to synthesize numerous factors affecting events in the bone-implant interface. Using the concept of surface area ratio allows us to study and compare such cellular responses to wear particles in a standardized manner.
在松动关节假体附近的肉芽肿组织的组织细胞/巨噬细胞中检测到颗粒状磨损碎片。通过用根据重量百分比、体积百分比和颗粒数量给药的颗粒刺激巨噬细胞，已在体外模拟了这种细胞与颗粒的相互作用。由于不同组成的刺激颗粒的大小和密度各不相同，这些给药方法中的每一种都有其固有的缺点。在本研究中，我们用二氧化钛和聚苯乙烯颗粒（<2微米）刺激P388D1巨噬细胞，给药基于颗粒表面积与细胞表面积的比率。确定了大小和组成对（1）骨吸收活性、（2）成纤维细胞增殖以及（3）白细胞介素-1和前列腺素E2分泌的影响。巨噬细胞对颗粒碎片的反应似乎取决于颗粒大小、组成以及由表面积比给出的剂量。用二氧化钛颗粒刺激的P388D1巨噬细胞释放白细胞介素-1，但不刺激成纤维细胞。在较高表面积比下巨噬细胞DNA合成的抑制表明细胞损伤或死亡。颗粒刺激的细胞使骨吸收增加至对照的125%，但仅释放基础水平的前列腺素E2。预计磨损颗粒刺激的巨噬细胞会合成多种影响骨-植入物界面事件的因子。使用表面积比的概念使我们能够以标准化方式研究和比较这种细胞对磨损颗粒的反应。
