人喉腺细胞磺基和唾液酸糖缀合物的细胞化学特征

Cytochemical characterization of sulfo- and sialoglycoconjugates of human laryngeal glandular cells.

作者信息

Castells M T, Madrid J F, Avilés M, Martínez-Menárguez J A, Ballesta J

机构信息

Department of Cell Biology, Medical School, University of Murcia, Spain.

出版信息

J Histochem Cytochem. 1994 Apr;42(4):485-96. doi: 10.1177/42.4.8126375.

DOI:10.1177/42.4.8126375

PMID:8126375

Abstract

The composition and distribution of sulfo- and sialoglycoconjugates in human laryngeal glands have been investigated at light and electron microscopic levels by use of peroxidase-, digoxigenin-, and colloidal gold-conjugated lectins in combination with several chemical and enzymatic deglycosylation procedures. The present study reveals a variety of terminal oligosaccharide sequences in serous and mucous glands. Serous cells contained glycoconjugates with terminal Neu5Ac (alpha 2-3) Gal (beta 1-4) GlcNAc, Neu5Ac (alpha 2-6) Gal/GalNAc, Neu5Ac (alpha 2-3/6) Gal (beta 1-3 GalNAc, GlcNAc, and Gal (beta 1-4) GlcNAc sequences. Scarce SO4Gal(beta 1-3)GalNAc terminal oligosaccharide chans were detected. Serous cells show wide morphological variability of secretory granules (electron lucent, electron dense, and bizonal) with different lectin affinities. Glycoconjugates in human laryngeal mucous glands contained a variety of terminal oligosaccharide sequences including SO4Gal(beta 1-4)GlcNAc, SO4Gal(beta 1-3) GalNAc, SO4GalNAc, and Neu5Ac(alpha 2-3)GalNAc.

摘要

利用过氧化物酶、地高辛和胶体金偶联凝集素，并结合多种化学和酶促去糖基化方法，在光镜和电镜水平上研究了人喉腺中磺基和唾液酸糖缀合物的组成和分布。本研究揭示了浆液性腺和黏液性腺中多种末端寡糖序列。浆液性腺细胞含有末端为Neu5Ac（α2-3）Gal（β1-4）GlcNAc、Neu5Ac（α2-6）Gal/GalNAc、Neu5Ac（α2-3/6）Gal（β1-3）GalNAc、GlcNAc和Gal（β1-4）GlcNAc序列的糖缀合物。检测到少量的SO4Gal（β1-3）GalNAc末端寡糖链。浆液性腺细胞显示出具有不同凝集素亲和力的分泌颗粒（电子透明、电子致密和双区）的广泛形态变异性。人喉黏液性腺中的糖缀合物包含多种末端寡糖序列，包括SO4Gal（β1-4）GlcNAc、SO4Gal（β1-3）GalNAc、SO4GalNAc和Neu5Ac（α2-3）GalNAc。