Creanor J, May J W, Mitchison J M
Eur J Biochem. 1975 Dec 15;60(2):487-93. doi: 10.1111/j.1432-1033.1975.tb21027.x.
The effect of 8-hydroxyquinoline, a rapid inhibitor of RNA synthesis, was followed on the activity of a number of enzymes in cultures of the fission yeast Schizosaccharomyces pombe. Two types of effect were found. In the first the activity continued to rise for a period and then remained constant. This occurred with alkaline phosphatase, basal and derepressed acid phosphatase, hexokinase, and derepressed sucrase and maltase at low cell density. It is consistent with control being exercised by an unstable mRNA or by an unstable stimulator of translocation. In the second the activity increased above the control values for several hours. This occurred with basal sucrase and maltase, and suggests a stable mRNA and an unstable inhibitor of translation. The extent of 'superproduction' of sucrase varied with cell density and with growth medium and this may be due to differences in the degree of translational inhibition. The possiblilty of a stable mRNA has interesting implications for the control of enzyme synthesis through the cell cycle.
研究了RNA合成的快速抑制剂8-羟基喹啉对裂殖酵母粟酒裂殖酵母培养物中多种酶活性的影响。发现了两种类型的影响。第一种情况是，活性在一段时间内持续上升，然后保持恒定。在低细胞密度下，碱性磷酸酶、基础型和去阻遏型酸性磷酸酶、己糖激酶以及去阻遏型蔗糖酶和麦芽糖酶均出现这种情况。这与由不稳定的mRNA或不稳定的转位刺激物进行调控相一致。第二种情况是，活性在数小时内高于对照值。基础型蔗糖酶和麦芽糖酶出现这种情况，这表明存在稳定的mRNA和不稳定的翻译抑制剂。蔗糖酶“超量产生”的程度随细胞密度和生长培养基而变化，这可能是由于翻译抑制程度的差异所致。稳定mRNA的可能性对于通过细胞周期控制酶合成具有有趣的意义。
