酵母细胞色素c的葡萄糖阻遏动力学
Kinetics of glucose repression of yeast cytochrome c.
作者信息
Zitomer R S, Nichols D L
出版信息
J Bacteriol. 1978 Jul;135(1):39-44. doi: 10.1128/jb.135.1.39-44.1978.
Abstract
The kinetics of glucose repression of cytochrome c synthesis was measured by a radioimmune assay. When 5 or 10% glucose was added to a derepressed culture, the rate of cytochrome c synthesis was reduced to the repressed level with a half-life of 2 min. The addition of 1 or 0.5% glucose repressed the rate of cytochrome c synthesis to the same level as high glucose concentrations but with a longer half-life of 3 min. Glucose repression had no effect on the stability or function of the cytochrome c protein. Cellular levels of active cytochrome c mRNA during glucose repression were measured by translation of total cellular polyadenylic acid-containing RNA and immunoprecipitation cytochrome c from the translation products. The results of these measurements indicate that glucose represses the rate of cytochrome c synthesis through a reduction in the level of translatable cytochrome c mRNA.
摘要
通过放射免疫测定法测定了葡萄糖对细胞色素c合成的阻遏动力学。当向去阻遏培养物中添加5%或10%的葡萄糖时，细胞色素c的合成速率降至阻遏水平，半衰期为2分钟。添加1%或0.5%的葡萄糖可将细胞色素c的合成速率阻遏至与高葡萄糖浓度相同的水平，但半衰期更长，为3分钟。葡萄糖阻遏对细胞色素c蛋白的稳定性或功能没有影响。在葡萄糖阻遏期间，通过翻译总细胞含聚腺苷酸的RNA并从翻译产物中免疫沉淀细胞色素c来测量活性细胞色素c mRNA的细胞水平。这些测量结果表明，葡萄糖通过降低可翻译的细胞色素c mRNA水平来阻遏细胞色素c的合成速率。
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