单个染色体的甲基化分析：亚硫酸氢盐基因组测序的改进方案 - Suppr

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超能文献

Methylation analysis on individual chromosomes: improved protocol for bisulphite genomic sequencing.

作者信息

Feil R, Charlton J, Bird A P, Walter J, Reik W

机构信息

Laboratory of Developmental Genetics and Imprinting, AFRC Babraham Institute, Cambridge, UK.

出版信息

Nucleic Acids Res. 1994 Feb 25;22(4):695-6. doi: 10.1093/nar/22.4.695.

DOI:10.1093/nar/22.4.695

PMID:8127720

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307863/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4991/307863/d039cf177f0e/nar00028-0155-a.jpg

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本文引用的文献

Reaction of sodium bisulfite with uracil, cytosine, and their derivatives.

Biochemistry. 1970 Jul 7;9(14):2858-65. doi: 10.1021/bi00816a016.

Deamination of cytosine derivatives by bisulfite. Mechanism of the reaction.

J Am Chem Soc. 1974 Feb 6;96(3):906-12. doi: 10.1021/ja00810a043.

A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827-31. doi: 10.1073/pnas.89.5.1827.

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

Proc Natl Acad Sci U S A. 1978 May;75(5):2170-4. doi: 10.1073/pnas.75.5.2170.

Bisulfite modification of nucleic acids and their constituents.

Prog Nucleic Acid Res Mol Biol. 1976;16:75-124. doi: 10.1016/s0079-6603(08)60756-4.

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