Grigg G W
Peptide Technology Limited, at the CSIRO Laboratory of Molecular Biology, Sydney, Australia.
DNA Seq. 1996;6(4):189-98. doi: 10.3109/10425179609008443.
Measuring patterns of cytosine methylation in genomic DNA is most efficiently accomplished by use of the bisulphite method. This method depends on the large difference in reactivity of cytosines relative to 5-Methyl cytosines in genomic DNA to bisulphite. The chemistry and history of the method and recent developments which greatly increase its sensitivity, simplicity and reliability are described. An updated protocol to guide users is appended.
通过使用亚硫酸氢盐法能最有效地实现对基因组DNA中胞嘧啶甲基化模式的检测。该方法依赖于基因组DNA中胞嘧啶与5-甲基胞嘧啶对亚硫酸氢盐反应性的巨大差异。本文描述了该方法的化学原理、发展历程以及近期极大提高其灵敏度、简便性和可靠性的进展。附录中附有一份供用户参考的更新方案。
