通过聚合酶链反应检测红霉素耐药决定簇。
Detection of erythromycin-resistant determinants by PCR.
作者信息
Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L
机构信息
Department of Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA.
出版信息
Antimicrob Agents Chemother. 1996 Nov;40(11):2562-6. doi: 10.1128/AAC.40.11.2562.
Abstract
Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.
摘要
红霉素耐药决定因素包括Erm甲基化酶、外排泵和灭活酶。为了区分临床分离株中不同的耐药机制，设计了PCR引物，以便在多重PCR中检测部分基因产物的扩增。这种方法能够对扩增的PCR产物进行直接测序，可用于比较临床菌株中的耐药决定因素。此外，这种方法在红霉素耐药决定因素的监测研究中可能会有用。
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