Guedes Barbosa L S, Gilbrut B, Shoenfeld Y, Scheinberg M A
Instituto do Cancer "Arnaldo Vierira de Carvalho", Instituto Brasileiro de Controle do Cancer, Sao Paulo, Brazil
Clin Rheumatol. 1996 Jan;15(1):26-8. doi: 10.1007/BF02231680.
Our objective was to assess the prevalence of autoantibodies in patients with leprosy. Forty-one cases of lepromatous leprosy were studied. For the detection of autoantibodies we used the Elisa technique using the following purified antigens in an Elisa assay: dsDNA, ssDNA, histone, mitochondria, RNA, RNP, SS-A, SS-B, Sm, Scl-70, Anca C, Anca P and the cardiolipin complex. As a "cut off" point we used values shown on previous studies to differentiate normal from elevated values. Antibodies to SS-B, mitochondria and cardiolipin were the most prevalent in our study. Antimitochondrial antibodies distinct from those seen in primary biliary cirrhosis and antiphospholipid antibodies with variable ligand activity to B2GIP are frequent in the sera of leprosy patients.
我们的目的是评估麻风病患者自身抗体的患病率。研究了41例瘤型麻风病例。为检测自身抗体，我们在酶联免疫吸附测定（ELISA）中使用ELISA技术，采用以下纯化抗原：双链DNA、单链DNA、组蛋白、线粒体、RNA、核糖核蛋白、SS - A、SS - B、Sm、Scl - 70、抗中性粒细胞胞浆抗体C型、抗中性粒细胞胞浆抗体P型和心磷脂复合物。作为“临界值”，我们使用先前研究中显示的值来区分正常与升高的值。在我们的研究中，抗SS - B、线粒体和心磷脂的抗体最为普遍。与原发性胆汁性肝硬化中所见不同的抗线粒体抗体以及对β2糖蛋白具有可变配体活性的抗磷脂抗体在麻风病患者血清中很常见。
