Division of Science and Food Technology, Faculty of Engineering and Agro-Industry, Maejo University, Chiang Mai 50290, Thailand.
Oxidative Stress Research Cluster, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Molecules. 2021 Nov 15;26(22):6887. doi: 10.3390/molecules26226887.
Rice grass has been reported to contain bioactive compounds that possess antioxidant and free-radical scavenging activities. We aimed to assess rice grass extract (RGE) drink by determining catechin content, free-radical scavenging and iron-binding properties, as well as toxicity in cells and animals. Young rice grass (Sukhothai-1 strain) was dried, extracted with hot water and lyophilized in a vacuum chamber. The resulting extract was reconstituted with deionized water (260 mg/40 mL) and served as Sukhothai-1 rice grass extract drink (ST1-RGE). HPLC results revealed at least eight phenolic compounds, for which the major catechins were catechin, epicatechin and epigallocatechin-3-gallate (EGCG) (2.71-3.57, 0.98-1.85 and 25.47-27.55 mg/40 mL serving, respectively). Elements (As, Cu, Pb, Sn and Zn) and aflatoxin (B1, B2, G1 and G2) contents did not exceed the relevant limits when compared with WHO guideline values. Importantly, ST1-RGE drink exerted radical-scavenging, iron-chelating and anti-lipid peroxidation properties in aqueous and biological environments in a concentration-dependent manner. The drink was not toxic to cells and animals. Thus, Sukhothai-1 rice grass product is an edible drink that is rich in catechins, particularly EGCG, and exhibited antioxidant, free radical scavenging and iron-binding/chelating properties. The product represents a functional drink that is capable of alleviating conditions of oxidative stress and iron overload.
糙叶红米草被报道含有具有抗氧化和清除自由基活性的生物活性化合物。我们旨在通过测定儿茶素含量、自由基清除和铁结合特性以及细胞和动物毒性来评估糙叶红米草提取物（RGE）饮料。将年轻的糙叶红米草（苏可泰-1 株）干燥，用热水提取并在真空中冻干。所得提取物用去离子水（260mg/40mL）复溶，作为苏可泰-1 红米草提取物饮料（ST1-RGE）。HPLC 结果显示至少有八种酚类化合物，其中主要的儿茶素有儿茶素、表儿茶素和表没食子儿茶素-3-没食子酸酯（EGCG）（分别为 2.71-3.57、0.98-1.85 和 25.47-27.55mg/40mL ）。与世界卫生组织指导值相比，元素（砷、铜、铅、锡和锌）和黄曲霉毒素（B1、B2、G1 和 G2）含量没有超过相关限制。重要的是，ST1-RGE 饮料在水相和生物环境中以浓度依赖的方式表现出自由基清除、铁螯合和抗脂质过氧化作用。该饮料对细胞和动物没有毒性。因此，苏可泰-1 红米草产品是一种富含儿茶素，特别是 EGCG 的可食用饮料，具有抗氧化、清除自由基和铁结合/螯合特性。该产品是一种功能性饮料，能够缓解氧化应激和铁过载的状况。
