Kawamoto M
Kawamoto Gynecological Clinic, Hiroshima, Japan.
Cancer. 1994 Apr 1;73(7):1836-41. doi: 10.1002/1097-0142(19940401)73:7<1836::aid-cncr2820730710>3.0.co;2-7.
The diagnosis of breast cancer based on nipple discharge, often the only clinical manifestation of early breast cancer, is currently unsatisfactory. Because M subunits of lactate dehydrogenase (LDH) have been noted to increase in cancer tissue, the author assessed the value of using LDH isozyme patterns in nipple discharge for the diagnosis of breast cancer.
LDH isozyme levels in (1) nipple discharge of patients diagnosed as having breast cancer, intraductal papilloma, mastopathy, drug-induced nipple discharge, mastitis, or benign nipple discharge; (2) control samples of normal nipple discharge (milk) 6 days, 1-5 months, and 6 months to 2 years postpartum; (3) the serum of patients presenting with nipple discharge; and (4) normal and cancerous breast tissue extracts were measured using a Ciba-Corning LDH isozyme system (Ciba Corning Diagnostic Corp., Tokyo, Japan).
LDH isozyme levels in the nipple discharge of patients with benign breast diseases displayed various patterns. Levels in the nipple discharge of patients with breast cancer, including noninvasive carcinoma, tended to increase in ascending order from LDH1 to LDH5. This pattern was similar to that in breast cancer tissue and was unrelated to the pattern in serum.
LDH isozyme assay of nipple discharge may be a useful technique for providing a supporting diagnosis of breast cancer.
基于乳头溢液来诊断乳腺癌（乳头溢液常常是早期乳腺癌的唯一临床表现），目前并不令人满意。由于已注意到乳酸脱氢酶（LDH）的M亚基在癌组织中会增加，作者评估了利用乳头溢液中LDH同工酶谱来诊断乳腺癌的价值。
采用日本东京的汽巴-康宁LDH同工酶系统（汽巴-康宁诊断公司）测量以下样本中的LDH同工酶水平：（1）被诊断患有乳腺癌、导管内乳头状瘤、乳腺病、药物性乳头溢液、乳腺炎或良性乳头溢液患者的乳头溢液；（2）产后6天、1至5个月以及6个月至2年的正常乳头溢液（乳汁）对照样本；（3）出现乳头溢液患者的血清；（4）正常和癌性乳腺组织提取物。
良性乳腺疾病患者乳头溢液中的LDH同工酶水平呈现出多种模式。包括非浸润性癌在内的乳腺癌患者乳头溢液中的水平往往从LDH1到LDH5呈升序增加。这种模式与乳腺癌组织中的模式相似，且与血清中的模式无关。
乳头溢液的LDH同工酶检测可能是一种有助于支持乳腺癌诊断的有用技术。
