未代谢的3H-7,12-二甲基苯并(a)蒽在再生大鼠肝脏中的持久性。
The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
作者信息
Tomsak R L, Cook R T
出版信息
Br J Cancer. 1975 Oct;32(4):440-50. doi: 10.1038/bjc.1975.245.
The hepatic subcellular distribution, binding and persistence of 3H-7,12-dimethylbenz(a)anthracene were compared in partially hepatectomized rats and in intact controls. By 2 weeks after injection, intact liver homogenates contained only 9% of the total radioactivity present 4 h after injection; regenerated liver contained 60% in spite of a tripling in liver mass during this time. Cell fractions isolated from regenerated liver had 9-59 fold greater hexane extractable specific activities than those from intact liver. The radioactivity present in hexane extracts co-chromatographed with a 3H-7,12-dimethylbenz(a)anthracene standard. Preliminary experiments demonstrated that liver microsomes isolated from DMBA treated partially hepatectomized animals metabolized less DMBA in vitro than did microsomes isolated from DMBA treated intact animals. The greater persistence of unmetabolized DMBA may be related to the greater carcinogenicity of this compound for regenerating, as compared with intact, rat liver.
比较了部分肝切除大鼠和完整对照大鼠中3H-7,12-二甲基苯并(a)蒽的肝脏亚细胞分布、结合和持久性。注射后2周，完整肝脏匀浆中仅含注射后4小时存在的总放射性的9%；尽管在此期间肝脏质量增加了两倍，但再生肝脏中含有60%。从再生肝脏分离的细胞组分的己烷可提取比活性比从完整肝脏分离的细胞组分高9至59倍。己烷提取物中存在的放射性与3H-7,12-二甲基苯并(a)蒽标准品共色谱。初步实验表明，从经二甲基苯并蒽处理的部分肝切除动物分离的肝微粒体在体外代谢的二甲基苯并蒽比从经二甲基苯并蒽处理的完整动物分离的微粒体少。与完整大鼠肝脏相比，未代谢的二甲基苯并蒽在再生肝脏中持续时间更长，这可能与其对再生肝脏更大的致癌性有关。
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