Baquar N, Threlfall E J, Rowe B, Stanley J
NCTC Molecular Genetics Unit, Central Public Health Laboratory, London, UK.
FEMS Microbiol Lett. 1994 Jan 15;115(2-3):291-5. doi: 10.1111/j.1574-6968.1994.tb06653.x.
The common phage type 193 of Salmonella typhimurium was analyzed with respect to molecular markers of chromosomal genotype. Three profiles of the 16S rRNA genes and seven profiles of the DNA insertion element IS200 were found among ten representative strains of DT193. The IS200 profiles found within this single phage type were highly diverse, confirming that DT193 is a composite phage type containing several distinct clones and hybrid lines. IS200 profiling is thus appropriate both for primary strain discrimination, and for subdivision within certain phage types of S. typhimurium, such as DT193. This rapid molecular definition of clonality will be useful for the epidemiological investigation of food poisoning outbreaks.
对鼠伤寒沙门氏菌常见的193型噬菌体进行了染色体基因型分子标记分析。在10株DT193代表性菌株中发现了16S rRNA基因的三种图谱和DNA插入元件IS200的七种图谱。在这种单一噬菌体类型中发现的IS200图谱高度多样，证实DT193是一种包含多个不同克隆和杂交系的复合噬菌体类型。因此，IS200图谱分析既适用于菌株的初步鉴别，也适用于鼠伤寒沙门氏菌某些噬菌体类型（如DT193）的细分。这种对克隆性的快速分子定义将有助于食物中毒暴发的流行病学调查。
