Jackson J K, Murphree R L, Tamplin M L
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611-0310, USA.
J Clin Microbiol. 1997 Aug;35(8):2098-101. doi: 10.1128/jcm.35.8.2098-2101.1997.
Vibrio vulnificus is the leading cause of food-related mortality reported in the state of Florida. It is normal microflora in marine environments, where seawater and molluscan shellfish are the primary vectors of V. vulnificus disease. Risk correlates with seasonally high numbers of V. vulnificus bacteria during the summer months. Currently, the infectious dose for humans, as well as whether the disease is caused by single or multiple strains found in molluscan shellfish, is unknown. In this work, we studied pulsed-field gel electrophoresis profiles of V. vulnificus strains isolated from blood and oysters associated with V. vulnificus disease. Results showed that ca. 10(3) V. vulnificus bacteria/gram of oyster and higher concentrations were associated with human infections and that a single V. vulnificus strain, evidenced by pulsed-field gel electrophoresis profiles, was isolated from human tissues.
创伤弧菌是佛罗里达州报告的与食物相关死亡的主要原因。它是海洋环境中的正常微生物群落，海水和软体贝类是创伤弧菌病的主要传播媒介。风险与夏季创伤弧菌数量季节性增加相关。目前，人类的感染剂量以及该疾病是否由软体贝类中发现的单一菌株或多种菌株引起尚不清楚。在这项工作中，我们研究了从与创伤弧菌病相关的血液和牡蛎中分离出的创伤弧菌菌株的脉冲场凝胶电泳图谱。结果表明，每克牡蛎中约10³个创伤弧菌及更高浓度与人类感染有关，并且通过脉冲场凝胶电泳图谱证明，从人体组织中分离出了单一的创伤弧菌菌株。
