Shrestha S M, Tsuda F, Okamoto H, Tokita H, Horikita M, Tanaka T, Miyakawa Y, Mayumi M
Liver Unit, Bir Hospital, Kathmandu, Nepal.
Hepatology. 1994 Apr;19(4):805-9.
A total of 145 patients with chronic liver disease, including 20 with chronic hepatitis, 63 with cirrhosis and 62 with primary hepatocellular carcinoma from Nepal were tested for markers of hepatitis B virus or hepatitis C virus infection. HBsAg was detected in 57 (39%) and hepatitis C virus RNA in 12 (8%); the cause of liver disease was not known in the remaining 76 (52%). HBsAg was found in 5 (1.3%) of 379 normal controls, whereas hepatitis C virus-associated antibodies were detected in 13 (3.4%), none of whom was positive for serum hepatitis C virus RNA. Subtypes of 102 HBsAg samples, from patients and asymptomatic carriers, were adw in 35 (34%), adr in 4 (4%) and ayw in 48 (47%); the remaining 15 (15%) were of atypical subtypes such as ad, ay and a. Of 12 hepatitis C virus RNA samples, genotype I was detected in 1, genotype II in 5 and genotype V in 1; the remaining five samples were not to be classified by polymerase chain reaction with primers specific for genotypes I to V deduced from hepatitis C virus core sequences, despite high hepatitis C virus RNA titers in all of them. Sequences of 192 amino acids in the entire E1 region of unclassifiable hepatitis C virus isolates from five patients differed from each other in 17% to 23%, and varied from reported isolates of defined genotypes in 13% to 44%. These results indicate that atypical subtypes of hepatitis B virus and novel genotypes of hepatitis C virus would prevail in Nepal.
对来自尼泊尔的145例慢性肝病患者进行了检测，其中包括20例慢性肝炎患者、63例肝硬化患者和62例原发性肝细胞癌患者，检测其乙型肝炎病毒或丙型肝炎病毒感染标志物。57例（39%）检测到乙肝表面抗原（HBsAg），12例（8%）检测到丙型肝炎病毒RNA；其余76例（52%）肝病病因不明。在379例正常对照中，5例（1.3%）检测到HBsAg，13例（3.4%）检测到丙型肝炎病毒相关抗体，但均无血清丙型肝炎病毒RNA阳性。来自患者和无症状携带者的102份HBsAg样本的亚型中，adw型35份（34%），adr型4份（4%），ayw型48份（47%）；其余15份（15%）为非典型亚型，如ad、ay和a型。在12份丙型肝炎病毒RNA样本中，1份检测到基因型I，5份检测到基因型II，1份检测到基因型V；其余5份样本尽管丙型肝炎病毒RNA滴度均很高，但用根据丙型肝炎病毒核心序列推导的I至V型特异性引物进行聚合酶链反应无法分型。来自5例患者的无法分型的丙型肝炎病毒分离株E1区全长192个氨基酸的序列彼此间差异为17%至23%，与已报道的确定基因型分离株的差异为13%至44%。这些结果表明，尼泊尔乙型肝炎病毒的非典型亚型和丙型肝炎病毒的新基因型较为普遍。
