Introduction of arbitrary sequences into genes by use of class IIs restriction enzymes.
作者信息
Beck R, Burtscher H
机构信息
Boehringer Mannheim Research Center, Penzberg, Germany.
出版信息
Nucleic Acids Res. 1994 Mar 11;22(5):886-7. doi: 10.1093/nar/22.5.886.
Abstract
摘要
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Introduction of arbitrary sequences into genes by use of class IIs restriction enzymes.利用IIs类限制酶将任意序列导入基因。
Nucleic Acids Res. 1994 Mar 11;22(5):886-7. doi: 10.1093/nar/22.5.886.
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Recognition of DNA by type II restriction enzymes.II型限制性内切酶对DNA的识别
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本文引用的文献
1
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.无需使用限制性内切酶构建杂交基因：通过重叠延伸进行基因剪接
Gene. 1989 Apr 15;77(1):61-8. doi: 10.1016/0378-1119(89)90359-4.
2
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.利用聚合酶链反应通过重叠延伸进行定点诱变。
Gene. 1989 Apr 15;77(1):51-9. doi: 10.1016/0378-1119(89)90358-2.
3
Class-IIS restriction enzymes--a review.IIS类限制酶——综述
Gene. 1991 Apr;100:13-26. doi: 10.1016/0378-1119(91)90345-c.
4
Fusion tails for the recovery and purification of recombinant proteins.用于重组蛋白回收和纯化的融合尾。
Protein Expr Purif. 1991 Apr-Jun;2(2-3):95-107. doi: 10.1016/1046-5928(91)90057-p.
5
A simple and rapid method for generating a deletion by PCR.一种通过聚合酶链式反应（PCR）产生缺失的简单快速方法。
Nucleic Acids Res. 1991 May 25;19(10):2785. doi: 10.1093/nar/19.10.2785.
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