Lehrer S B, Rose T M, Vaughan J H
Immunology. 1976 Jan;30(1):107-15.
Reaginic antibody in ascitic fluid was isolated from mice immunized with ovalbumin and TAl(OH)3 or with avalbumin and pertussis adjuvant. Partial purification of this reagin by ammonium sulphate precipitation, gel filtration, and ion-exchange column chromatography yielded a gammaglobulin fraction highly active in the passive cutaneous anaphylaxis reaction. This was identical in purity and specific activity to a fraction of partially purified reagin isolated from mouse antiserum. The recovery of reaginic activity after purification from ascitic fluid was 19 per cent compared to the 7-8 per cent recovery from anti-serum. These studies demonstrate that ascitic fluid in mice immunized with methods known to produce reaginic antibody provides an excellent source of mouse IgE.
从用卵清蛋白和氢氧化铝或卵清蛋白和百日咳佐剂免疫的小鼠腹水中分离出反应素抗体。通过硫酸铵沉淀、凝胶过滤和离子交换柱色谱对该反应素进行部分纯化，得到在被动皮肤过敏反应中具有高活性的γ球蛋白组分。其纯度和比活性与从小鼠抗血清中分离出的部分纯化反应素组分相同。从腹水中纯化后反应素活性的回收率为19%，而从抗血清中纯化的回收率为7-8%。这些研究表明，用已知能产生反应素抗体的方法免疫的小鼠腹水是小鼠IgE的极好来源。
