Sperduto A R, Bryson Y J, Chen I S
Department of Pediatrics, UCLA School of Medicine 90024.
AIDS Res Hum Retroviruses. 1993 Dec;9(12):1277-85. doi: 10.1089/aid.1993.9.1277.
The relative susceptibility of neonatal/cord blood monocyte/macrophages to productive infection with human immunodeficiency virus type 1 (HIV-1) was investigated. In addition, the effect of HIV-1 infection of cord blood monocyte/macrophages in various stages of maturation/differentiation as represented by differing ages of monocytes in culture was examined. Monocyte/macrophages were infected with two viral strains isolated and cloned from primary clinical isolates, each with different cell tropisms. Cord blood and adult monocyte/macrophages were infected with either the macrophage-tropic strain HIV-1(JR-FL) or the predominantly lymphocyte-tropic strain HIV-1(JR-CSF). p24gag antigen levels were measured in supernatants by ELISA. Cord monocyte/macrophages at three different ages in culture (4, 7, and 11 days) were more productively infected by both viral strains than were adult monocyte/macrophages infected in parallel. In addition, the less differentiated cells (cord and adult monocyte/macrophages infected after growing 4 days in culture) were more productively infected than were the more differentiated monocyte/macrophages (cells infected after growing 7 or 11 days in culture). The mechanism for this increased susceptibility of cord monocyte/macrophages to HIV-1 infection as compared to adult cells was also investigated. A measurable increase in DNA synthesis was found in the infected cord cells when compared to infected adult cells and to uninfected adult or cord cells as represented by increased [3H]thymidine incorporation, suggesting that increased cell proliferation of cord monocyte/macrophages may enhance the permissivity of infection. This article suggests that cord monocyte/macrophages may play an important role in the pathogenesis of perinatal HIV-1 infection.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了新生儿/脐带血单核细胞/巨噬细胞对1型人类免疫缺陷病毒（HIV-1）产生感染的相对易感性。此外，还检测了培养中不同年龄单核细胞所代表的脐带血单核细胞/巨噬细胞在成熟/分化各阶段感染HIV-1的影响。单核细胞/巨噬细胞用从原发性临床分离株中分离和克隆的两种病毒株感染，每种病毒株具有不同的细胞嗜性。脐带血和成人单核细胞/巨噬细胞用巨噬细胞嗜性病毒株HIV-1（JR-FL）或主要淋巴细胞嗜性病毒株HIV-1（JR-CSF）感染。通过ELISA测量上清液中的p24gag抗原水平。培养中三个不同年龄（4、7和11天）的脐带单核细胞/巨噬细胞比平行感染的成人单核细胞/巨噬细胞更易被两种病毒株有效感染。此外，分化程度较低的细胞（培养4天后感染的脐带和成人单核细胞/巨噬细胞）比分化程度较高的单核细胞/巨噬细胞（培养7或11天后感染的细胞）更易被有效感染。还研究了与成人细胞相比，脐带单核细胞/巨噬细胞对HIV-1感染易感性增加的机制。与感染的成人细胞以及未感染的成人或脐带细胞相比，感染的脐带细胞中DNA合成有可测量的增加，表现为[3H]胸苷掺入增加，这表明脐带单核细胞/巨噬细胞细胞增殖增加可能增强了感染的易感性。本文表明脐带单核细胞/巨噬细胞可能在围产期HIV-1感染的发病机制中起重要作用。（摘要截短为250字）
