广宿主范围温和型链霉菌噬菌体phi HAU3的特性及噬菌粒的构建
Characterization of phi HAU3, a broad-host-range temperate streptomyces phage, and development of phasmids.
作者信息
Zhou X, Deng Z, Hopwood D A, Kieser T
机构信息
Department of Soil Sciences and Agrochemistry, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
出版信息
J Bacteriol. 1994 Apr;176(7):2096-9. doi: 10.1128/jb.176.7.2096-2099.1994.
Abstract
phi HAU3 is a temperate Streptomyces phage with cohesive ends and a broad host range that includes Streptomyces hygroscopicus 10-22, a producer of antifungal compounds, but it fails to grow on Streptomyces lividans 66. Two phasmid derivatives were constructed that function as lambda cosmid vectors in Escherichia coli and as phages in Streptomyces spp.
摘要
phi HAU3是一种具有粘性末端和广泛宿主范围的温和型链霉菌噬菌体，其宿主范围包括产抗真菌化合物的吸水链霉菌10-22，但不能在变铅青链霉菌66上生长。构建了两种噬菌粒衍生物，它们在大肠杆菌中作为λ粘粒载体起作用，在链霉菌属中作为噬菌体起作用。
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