携带链霉菌噬菌体phi C31 cos位点的穿梭载体的构建、转导及其粘性末端的测定。

Construction and transduction of a shuttle vector bearing the cos site of Streptomyces phage phi C31 and determination of its cohesive ends.

作者信息

Kobler L, Schwertfirm G, Schmieger H, Bolotin A, Sladkova I

机构信息

Institut für Genetik and Mikrobiologie Universität München, F.R.G.

出版信息

FEMS Microbiol Lett. 1991 Mar 1;62(2-3):347-53. doi: 10.1016/0378-1097(91)90183-b.

DOI:10.1016/0378-1097(91)90183-b

PMID:2040440

Abstract

A plasmid has been constructed which is a bifunctional vector for Escherichia coli and for streptomycetes, containing the cos site of Streptomyces phage phi C31. It can be efficiently transduced into S. lividans 66 and into several other Streptomyces species. The nucleotide sequence of a 311-bp DNA fragment containing the cohesive ends has been determined. The cohesive ends consist of 10 bases protruding at the 3'-end of the phage genome.

摘要

构建了一种质粒，它是用于大肠杆菌和链霉菌的双功能载体，含有链霉菌噬菌体φC31的粘性末端位点。它可以高效转导到变铅青链霉菌66及其他几种链霉菌中。已确定了包含粘性末端的311bp DNA片段的核苷酸序列。粘性末端由噬菌体基因组3'端突出的10个碱基组成。