枯草芽孢杆菌的诱导型琥珀抑制基因
Inducible amber suppressor for Bacillus subtilis.
作者信息
Grundy F J, Henkin T M
机构信息
Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
出版信息
J Bacteriol. 1994 Apr;176(7):2108-10. doi: 10.1128/jb.176.7.2108-2110.1994.
Abstract
An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.
摘要
构建了枯草芽孢杆菌酪氨酰 - tRNA的琥珀抑制变体，并将其置于异丙基 - β - D - 硫代半乳糖苷（IPTG）诱导型Pspac启动子的控制之下。添加IPTG导致含有UAG琥珀密码子的rpsD - lacZ融合蛋白的表达增加了50倍。该系统允许分离出一种条件致死突变体，其生长需要IPTG。
相似文献
1
Inducible amber suppressor for Bacillus subtilis.枯草芽孢杆菌的诱导型琥珀抑制基因
J Bacteriol. 1994 Apr;176(7):2108-10. doi: 10.1128/jb.176.7.2108-2110.1994.
2
Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis.基于IPTG诱导型启动子的枯草芽孢杆菌无诱导剂表达质粒的构建
Microb Cell Fact. 2017 Jul 25;16(1):130. doi: 10.1186/s12934-017-0747-0.
3
The amount of RepR protein determines the copy number of plasmid pIP501 in Bacillus subtilis.RepR蛋白的数量决定了枯草芽孢杆菌中质粒pIP501的拷贝数。
J Bacteriol. 1992 Aug;174(16):5475-8. doi: 10.1128/jb.174.16.5475-5478.1992.
4
Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells.利用 IPTG 诱导的 Pgrac212 启动子在枯草芽孢杆菌细胞质中过表达人鼻病毒 3C 蛋白酶融合蛋白。
Curr Microbiol. 2019 Dec;76(12):1477-1486. doi: 10.1007/s00284-019-01783-9. Epub 2019 Oct 14.
5
FtsZ and nucleoid segregation during outgrowth of Bacillus subtilis spores.枯草芽孢杆菌孢子萌发过程中的FtsZ与类核分离
J Bacteriol. 1995 May;177(9):2560-3. doi: 10.1128/jb.177.9.2560-2563.1995.
6
Influence of transcriptional and translational control sequences on the expression of foreign genes in Caulobacter crescentus.转录和翻译控制序列对新月柄杆菌中外源基因表达的影响。
J Bacteriol. 1994 May;176(9):2603-10. doi: 10.1128/jb.176.9.2603-2610.1994.
7
The 3' codon context effect on UAG suppressor tRNA is different in Escherichia coli and human cells.3'密码子上下文对UAG抑制性tRNA的影响在大肠杆菌和人类细胞中有所不同。
J Mol Biol. 1993 Sep 5;233(1):1-6. doi: 10.1006/jmbi.1993.1479.
8
The primary role of comA in establishment of the competent state in Bacillus subtilis is to activate expression of srfA.ComA在枯草芽孢杆菌感受态建立过程中的主要作用是激活srfA的表达。
J Bacteriol. 1991 Nov;173(22):7269-74. doi: 10.1128/jb.173.22.7269-7274.1991.
9
A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.一种基于枯草芽孢杆菌孢子形成途径的去阻遏系统可实现对异源基因表达的动态控制。
Appl Environ Microbiol. 2007 Apr;73(7):2390-3. doi: 10.1128/AEM.02266-06. Epub 2007 Feb 9.
10
Translational nonsense codon suppression as indicator for functional pre-tRNA splicing in transformed Arabidopsis hypocotyl-derived calli.翻译无义密码子抑制作为转化的拟南芥下胚轴来源愈伤组织中功能性前体tRNA剪接的指标。
Nucleic Acids Res. 2003 Feb 15;31(4):1197-207. doi: 10.1093/nar/gkg220.
引用本文的文献
1
Translational Control using an Expanded Genetic Code.利用扩展遗传密码进行翻译控制。
Int J Mol Sci. 2019 Feb 18;20(4):887. doi: 10.3390/ijms20040887.
2
Factors that influence T box riboswitch efficacy and tRNA affinity.影响T盒核糖开关效能和tRNA亲和力的因素。
Bioorg Med Chem. 2015 Sep 1;23(17):5702-8. doi: 10.1016/j.bmc.2015.07.018. Epub 2015 Jul 16.
3
High-throughput system for the presentation of secreted and surface-exposed proteins from Gram-positive bacteria in functional metagenomics studies.高通量系统用于革兰氏阳性菌分泌蛋白和表面暴露蛋白在功能宏基因组学研究中的展示。
PLoS One. 2013 Jun 14;8(6):e65956. doi: 10.1371/journal.pone.0065956. Print 2013.
4
tRNA determinants for transcription antitermination of the Bacillus subtilis tyrS gene.枯草芽孢杆菌tyrS基因转录抗终止的tRNA决定因素。
RNA. 2000 Aug;6(8):1131-41. doi: 10.1017/s1355838200992100.
5
Interaction between the acceptor end of tRNA and the T box stimulates antitermination in the Bacillus subtilis tyrS gene: a new role for the discriminator base.tRNA的受体端与T盒之间的相互作用刺激枯草芽孢杆菌tyrS基因中的抗终止作用：鉴别碱基的新作用。
J Bacteriol. 1994 Aug;176(15):4518-26. doi: 10.1128/jb.176.15.4518-4526.1994.
本文引用的文献
1
tRNA as a positive regulator of transcription antitermination in B. subtilis.转运RNA作为枯草芽孢杆菌中转录抗终止的正调控因子。
Cell. 1993 Aug 13;74(3):475-82. doi: 10.1016/0092-8674(93)80049-k.
2
The Bacillus subtilis ochre suppressor sup-3 is located in an operon of seven tRNA genes.枯草芽孢杆菌赭石色抑制基因sup-3位于一个由七个tRNA基因组成的操纵子中。
J Bacteriol. 1993 Oct;175(20):6512-7. doi: 10.1128/jb.175.20.6512-6517.1993.
3
4
Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.琥珀抑制：酪氨酸转运RNA反密码子中的核苷酸变化。
Nature. 1968 Mar 16;217(5133):1019-24. doi: 10.1038/2171019a0.
5
Suppressor system in Bacillus subtilis 168.枯草芽孢杆菌168中的抑制系统。
J Bacteriol. 1969 Mar;97(3):1397-402. doi: 10.1128/jb.97.3.1397-1402.1969.
6
Isolation of a suppressor mutant in Bacillus subtilis.枯草芽孢杆菌中一个抑制突变体的分离
J Bacteriol. 1968 Mar;95(3):1187-8. doi: 10.1128/jb.95.3.1187-1188.1968.
7
Catabolic repression of bacterial sporulation.细菌芽孢形成的分解代谢阻遏
Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11. doi: 10.1073/pnas.54.3.704.
8
Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression.枯草芽孢杆菌中一种芽孢形成σ因子的加工：形态结构如何控制基因表达。
Cell. 1988 Mar 11;52(5):697-704. doi: 10.1016/0092-8674(88)90407-2.
9
Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.转移核糖核酸介导的大肠杆菌终止密码子抑制作用。
Microbiol Rev. 1988 Sep;52(3):354-74. doi: 10.1128/mr.52.3.354-374.1988.
10
Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation.枯草芽孢杆菌突变等位基因sup-3导致在赭石密码子处插入赖氨酸：sup-3在翻译衰减研究中的应用。
J Bacteriol. 1989 Oct;171(10):5322-4. doi: 10.1128/jb.171.10.5322-5324.1989.
Zotero 插件