枯草芽孢杆菌孢子萌发过程中的FtsZ与类核分离
FtsZ and nucleoid segregation during outgrowth of Bacillus subtilis spores.
作者信息
Partridge S R, Wake R G
机构信息
Department of Biochemistry, University of Sydney, New South Wales, Australia.
出版信息
J Bacteriol. 1995 May;177(9):2560-3. doi: 10.1128/jb.177.9.2560-2563.1995.
Spores of a strain of Bacillus subtilis in which ftsZ was under the control of the spac promoter were allowed to germinate and grow out in the presence of increasing concentrations of isopropyl-beta-D-thiogalactopyranoside (IPTG). Over the IPTG concentration range of 0 to 10(-3) M, the level of FtsZ from the time when the first nucleoid segregations were occurring, measured in Western blot (immunoblot) transfer experiments, varied between 15 and 100% of that in the wild type. Septation was completely blocked (for at least several hours) when the amount of FtsZ was < 30% of the wild-type level. At all levels of ftsZ induction, the timing and rate of segregation of nucleoids following the first round of replication were unaltered. It is concluded that FtsZ has no direct role in nucleoid segregation in this situation.
使ftsZ受spac启动子控制的枯草芽孢杆菌菌株的孢子，在异丙基-β-D-硫代半乳糖苷（IPTG）浓度不断增加的情况下萌发并生长。在0至10⁻³ M的IPTG浓度范围内，在蛋白质免疫印迹（免疫印迹）转移实验中测量，从首次出现类核分离时起，FtsZ的水平在野生型水平的15%至100%之间变化。当FtsZ的量小于野生型水平的30%时，隔膜形成完全受阻（至少几个小时）。在ftsZ诱导的所有水平下，第一轮复制后类核分离的时间和速率均未改变。得出的结论是，在这种情况下，FtsZ在类核分离中没有直接作用。
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