Neutra M R, Kraehenbuhl J P
Department of Pediatrics, Harvard Medical School, Boston, MA 02115.
J Cell Sci Suppl. 1993;17:209-15. doi: 10.1242/jcs.1993.supplement_17.29.
Transepithelial transport of antigens by M cells in the epithelium associated with lymphoid follicles in the intestine delivers immunogens directly to organized mucosal lymphoid tissues, the inductive sites for mucosal immune responses. We have exploited M cell transport to generate and characterize specific monoclonal IgA antibodies that can prevent interaction of pathogens with epithelial surfaces. The relative protective capacities of specific monoclonal IgA antibodies have been tested in vivo by generation of hybridoma tumors that result in secretion of monoclonal IgA into the intestine. Using this method, we have established that secretion of IgA antibodies recognizing a single surface epitope on enteric pathogens can provide protection against colonization or invasion of the intestinal mucosa.
肠道中与淋巴滤泡相关的上皮内M细胞介导的抗原跨上皮转运可将免疫原直接递送至有组织的黏膜淋巴组织，即黏膜免疫反应的诱导部位。我们利用M细胞转运来产生和鉴定能够阻止病原体与上皮表面相互作用的特异性单克隆IgA抗体。通过产生能将单克隆IgA分泌到肠道中的杂交瘤肿瘤，在体内测试了特异性单克隆IgA抗体的相对保护能力。利用这种方法，我们证实分泌识别肠道病原体单一表面表位的IgA抗体可提供针对肠道黏膜定植或侵袭的保护作用。
