来自兔肠道派尔集合淋巴结的独特电生理特征。

A distinctive electrophysiological signature from the Peyer's patches of rabbit intestine.

作者信息

Brayden D J, Baird A W

机构信息

Elan Corporation Research Institute, Trinity College Dublin, Ireland.

出版信息

Br J Pharmacol. 1994 Oct;113(2):593-9. doi: 10.1111/j.1476-5381.1994.tb17031.x.

DOI:10.1111/j.1476-5381.1994.tb17031.x

PMID:7530575

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1510135/

Abstract

Rabbit small intestinal segments containing Peyer's patches (PP) were examined in Ussing chambers using short-circuit current (Isc) recording. By comparison with control small intestinal mucosal segments, rabbit PP-containing epithelia exhibited decreased basal Isc, increased transepithelial resistance (TER) and unchanged potential difference (PD). 2. Carbachol caused a decrease in Isc in rabbit PP epithelia. Forskolin, dibutyryl cyclic GMP, histamine and the calcium ionophore, A23187, were without effect. In contrast, control epithelial segments of rabbit intestine responded to carbachol and forskolin with an increased Isc, indicative of electrogenic chloride secretion. The EC50 for carbachol was approximately 2 microM in both types of epithelia. Methacholine also caused an outward current in rabbit PP epithelia which had similar properties to that of carbachol. The effect of the cholinomimetics on rabbit PP was basolateral-sided, reversible, and sensitive to low concentrations of the general muscarinic cholinoceptor blockers, atropine, scopolamine and also to the M1 cholinoceptor blocker, pirenzepine. 3. The Isc response to cholinomimetics in rabbit PP was insensitive to bumetanide, amiloride, TEA, barium, acetazolamide, piroxicam and omeprazole, but was attenuated in the presence of ouabain. Using bilaterally-substituted solutions, the carbachol effect on rabbit PP Isc was abolished in chloride/bicarbonate-free, but not in chloride-free solutions, suggestive of stimulation of electrogenic bicarbonate absorption by the agent. Substitution for sodium abolished both the basal current and the Isc response to carbachol. Part of the effect of carbachol on PP Isc appeared to be mediated by submucosal neurones because addition of tetrodotoxin reduced the effect by 60%. 4 As microfold (M) epithelial cells predominate in the PP of the rabbit, the unusual phenotype of cholinomimetic-induced outward current may be used as an electrophysiological marker for these potential sites of oral vaccine delivery, and in particular it may also be of use as a marker for rabbit M cells.

摘要

使用短路电流（Isc）记录法，在尤斯灌流小室中对含有派尔集合淋巴结（PP）的兔小肠段进行检测。与对照小肠黏膜段相比，含有兔PP的上皮组织基础Isc降低，跨上皮电阻（TER）增加，而电位差（PD）不变。2. 卡巴胆碱使兔PP上皮组织的Isc降低。福斯可林、二丁酰环磷鸟苷、组胺和钙离子载体A23187均无作用。相比之下，兔肠对照上皮段对卡巴胆碱和福斯可林的反应是Isc增加，表明是氯的电分泌。两种上皮组织中卡巴胆碱的半数有效浓度（EC50）约为2微摩尔。乙酰甲胆碱也使兔PP上皮组织产生外向电流，其性质与卡巴胆碱相似。拟胆碱药对兔PP的作用是从基底外侧开始的，可逆，且对低浓度的一般毒蕈碱型胆碱受体阻断剂阿托品、东莨菪碱以及M1胆碱受体阻断剂哌仑西平敏感。3. 兔PP对拟胆碱药的Isc反应对布美他尼、阿米洛利、四乙铵、钡、乙酰唑胺、吡罗昔康和奥美拉唑不敏感，但在哇巴因存在时减弱。使用双侧替代溶液，卡巴胆碱对兔PP Isc的作用在无氯/无碳酸氢盐溶液中被消除，但在无氯溶液中未被消除，提示该药物刺激了电生性碳酸氢盐吸收。用钠替代后，基础电流和对卡巴胆碱的Isc反应均被消除。卡巴胆碱对PP Isc的部分作用似乎是由黏膜下神经元介导的，因为加入河豚毒素可使该作用降低60%。4. 由于微褶（M）上皮细胞在兔PP中占主导地位，拟胆碱药诱导的外向电流这种不寻常的表型可作为这些口服疫苗递送潜在部位的电生理标记物，特别是它也可能用作兔M细胞的标记物。