Gabbianelli R, Kantar A, Oggiano N, Fiorini R, Falcioni G, Giorgi P L
Dipartimento di Biologia, Università degli Studi, Camerino, Macerata.
Minerva Pediatr. 1993 Dec;45(12):493-7.
In response to certain stimuli, polymorphonuclear leukocytes (PMNs) undergo an oxidative burst during which a series of reactive oxygen metabolites are generated. The importance of the release of these oxygen metabolites by polymorphonuclear leukocytes, is recognized to be a key event in the function of these cells during infection and inflammation. We have evaluated the release of reactive oxygen species during the activation of respiratory burst (RB) of PMNs obtained from children with trisomy 21 using chemiluminescence techniques. As chemiluminogenic probes we have employed lucigenin and luminon that are know to be sensitive to the superoxide anion and the H2O2-myeloperoxidase-halide system of PMNs, respectively. Activated PMNs from children with trisomy 21 exhibited a low level of superoxide and a reduced activity of H2O2-myeloperoxidase-halide system compared to the control group. No significant difference in extracellular H2O2 release was observed. It seems likely that alterations in the enzymatic activities of the Cu/Zn-Superoxide dismutase and myeloperoxidase induce imbalance in the release of reactive oxygen species in activated PMNs from children with trisomy 21. This imbalance could be on the basis of the increased oxidative injury reported in trisomy 21.
在某些刺激下，多形核白细胞（PMNs）会经历氧化爆发，在此期间会产生一系列活性氧代谢产物。多形核白细胞释放这些氧代谢产物的重要性，被认为是这些细胞在感染和炎症过程中发挥功能的关键事件。我们使用化学发光技术评估了从21三体综合征患儿获取的多形核白细胞呼吸爆发（RB）激活过程中活性氧的释放情况。作为化学发光探针，我们使用了光泽精和鲁米诺，已知它们分别对超氧阴离子和多形核白细胞的H2O2-髓过氧化物酶-卤化物系统敏感。与对照组相比，21三体综合征患儿的活化多形核白细胞表现出低水平的超氧阴离子和降低的H2O2-髓过氧化物酶-卤化物系统活性。未观察到细胞外H2O2释放的显著差异。21三体综合征患儿活化多形核白细胞中铜/锌超氧化物歧化酶和髓过氧化物酶的酶活性改变似乎可能导致活性氧释放失衡。这种失衡可能是21三体综合征中报道的氧化损伤增加的基础。
