Ferris D G, Martin W H, Mathis D M, Steele J C, Fischer P M, Styslinger K M
Department of Family Medicine, Medical College of Georgia, Augusta 30912.
J Fam Pract. 1991 Jul;33(1):73-8.
The purpose of this investigation was to evaluate the ability of a rapid enzyme immunoassay test to noninvasively detect Chlamydia trachomatis urethritis in men from a urine specimen.
Urethral samples and urine from 207 patients were evaluated. Urethral and urine sediment Gram stains, leukocyte esterase dipstick tests, and enzyme immunoassay analyses of centrifuged and uncentrifuged urine were compared with urethral C trachomatis culture.
The prevalence of infection in this population was 10.3%. Sensitivity and specificity of the enzyme immunoassay on the centrifuged urine specimen were 70% and 96%, respectively. The positive and negative predictive values were 67% and 97%, respectively. The uncentrifuged urine enzyme immunoassay sensitivity was 35.7% and specificity was 98.9%. Leukocyte esterase test sensitivity compared with that of the Neisseria gonorrhoeae and/or C trachomatis cultures was 83.3%, and specificity was 52%.
The rapid enzyme immunoassay clinically complemented the screening urine sediment Gram stain and the leukocyte esterase test. The judicious use of a noninvasive C trachomatis rapid enzyme immunoassay test to identify organism-specific urethritis may improve patient management of sexually transmitted disease.
本研究的目的是评估一种快速酶免疫测定试验从尿液标本中无创检测男性沙眼衣原体尿道炎的能力。
对207例患者的尿道样本和尿液进行评估。将尿道和尿液沉淀物革兰氏染色、白细胞酯酶试纸条试验以及对离心和未离心尿液的酶免疫测定分析与尿道沙眼衣原体培养进行比较。
该人群的感染率为10.3%。离心尿液标本酶免疫测定的敏感性和特异性分别为70%和96%。阳性和阴性预测值分别为67%和97%。未离心尿液酶免疫测定的敏感性为35.7%，特异性为98.9%。与淋病奈瑟菌和/或沙眼衣原体培养相比，白细胞酯酶试验的敏感性为83.3%，特异性为52%。
快速酶免疫测定在临床上补充了筛查尿液沉淀物革兰氏染色和白细胞酯酶试验。明智地使用无创沙眼衣原体快速酶免疫测定试验来识别病原体特异性尿道炎可能会改善性传播疾病患者的管理。
