Isobe T, Shimizu S, Yoshihara S, Suzuki K
Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Res Vet Sci. 1994 Jan;56(1):123-5. doi: 10.1016/0034-5288(94)90209-7.
To investigate the molecular weight of the schizont antigen of Leucocytozoon caulleryi, the schizont antigen was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot. Thirty to 35 protein bands were detected by SDS-PAGE under reduced condition and ranged from 25 to 300 kDa. Most of them reacted with the sera of infected chickens by Western blot. The bands corresponding to polypeptides of 44, 58, 67 and 180 kDa reacted strongly, however, the bands of 30, 50 and 78 kDa did not react.
为研究卡氏住白细胞虫裂殖体抗原的分子量，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹法对裂殖体抗原进行分析。在还原条件下，通过SDS-PAGE检测到30至35条蛋白带，分子量范围为25至300 kDa。通过蛋白质印迹法，其中大多数蛋白带与感染鸡的血清发生反应。对应于44、58、67和180 kDa多肽的条带反应强烈，然而，30、50和78 kDa的条带未发生反应。
