Jensen H E, Espinosa de los Monteros A, Carrasco L
Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
J Comp Pathol. 1996 Feb;114(2):183-91. doi: 10.1016/s0021-9975(96)80007-7.
Of 73 goats on a dairy farm, 27 developed mycotic mastitis in the post-partum period. Purulent mammary secretion, progressive induration of the affected glands, slight fever and weight loss were observed. As treatment produced no improvement within 2-3 weeks, all diseased animals were slaughtered. At post-mortem examination, widespread acute and chronic mycotic lesions were seen throughout the affected glands. Infection was thought to have spread through the milk ducts as an ascending infection resulting from prophylactic intramammary treatment with antibiotic before parturition. In all lesions, aspergillus hyphae were identified by indirect immunofluorescent labelling, which gave a strong and uniform reaction with polyclonal and monoclonal antibodies to Aspergillus fumigatus. In a single granulomatous lesion, zygomycotic hyphae were also identified immunohistochemically. At terminal swellings of aspergillus hyphae, "yeast-like bodies" were produced, a phenomenon which seems to be associated with special, but unknown, circumstances.
在一个奶牛场的73只山羊中，有27只在产后发生了霉菌性乳腺炎。观察到有脓性乳腺分泌物、患病腺体进行性硬结、低热和体重减轻。由于在2至3周内治疗没有改善，所有患病动物均被屠宰。尸检时，在整个患病腺体中可见广泛的急性和慢性霉菌病变。感染被认为是通过乳管传播的，是分娩前预防性乳房内注射抗生素导致的上行感染。在所有病变中，通过间接免疫荧光标记鉴定出曲霉菌丝，其与针对烟曲霉的多克隆和单克隆抗体产生强烈且均匀的反应。在单个肉芽肿性病变中，还通过免疫组织化学鉴定出接合菌菌丝。在曲霉菌丝的末端肿胀处，产生了“酵母样体”，这种现象似乎与特殊但未知的情况有关。
