Bratanov V, Lilkova N, Stoianova-Zaĭkova L
Vet Med Nauki. 1975;12(4):76-83.
Studied were pigs with experimental Trichinella spiralis infection by means of biopsy, the complement-fixation test, the tube precipitation reaction, the double diffusion technique after Ouchterlony, and the microimmunoelectrophoresis after Scheidegger, using an antigen obtained by the method of Tanner and Gregory as modified by the authors. Highest titers established by the c. f. test and the tube precipitation reaction were observed on 30th--60th day after the infection. Complement-fixing antibodies were found up to the end of the third month, and precipitins -- up to the end of the fourth month of the infection. With the use of the double diffusion technique and the immunoelectrophoresis method the precipitation arcs were observed in the 2nd and 3rd month after the infection.
通过活检、补体结合试验、试管沉淀反应、奥克特洛尼氏双扩散技术以及谢德格氏微量免疫电泳法,对感染旋毛虫的实验猪进行了研究,所用抗原是作者对坦纳和格雷戈里方法进行改进后获得的。补体结合试验和试管沉淀反应所确定的最高滴度在感染后第30至60天观察到。补体结合抗体在感染后第三个月末仍可检测到,沉淀素在感染后第四个月末仍可检测到。使用双扩散技术和免疫电泳法时,在感染后第2和3个月观察到沉淀弧。