Lilkova N
Vet Med Nauki. 1977;14(2):57-62.
The double diffusion method after Ouchterlony was employed for the diagnosis of swine trichinellosis. Studied were sera of 12 experimentally infected pigs from the 15th to the 365th day after their treatment with moderate and massive doses of Trichinella spiralis. The antigen used was an extract of freeze-dried decapsulated muscular trichinellae, obtained by Tanner and Gregory's method modified according to the institute's laboratory conditions. Results showed that Ouchterlony's double diffusion reaction is specific and can used for the diagnosis of trichinellosis in pigs within the period cited above. The reaction has proved to be most strongly expressed when used between the 30th and 120th day.
采用奥克特洛尼氏双扩散法诊断猪旋毛虫病。研究了12头经中等剂量和大剂量旋毛虫治疗后第15天至第365天的实验感染猪的血清。所用抗原是冻干脱囊肌肉旋毛虫提取物，按照该研究所实验室条件对坦纳和格雷戈里的方法进行修改后获得。结果表明，奥克特洛尼氏双扩散反应具有特异性，可用于上述时期猪旋毛虫病的诊断。已证明该反应在第30天至第120天之间使用时表现最为强烈。
