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[免疫荧光技术在猪旋毛虫病诊断中的应用。II. 与试管试验和冷冻切片法的比较研究(作者译)]

[Immunofluorescence technique in diagnosis of trichinellosis in swine. II. Comparative investigations with the tube test and the cryostate method (author's transl)].

作者信息

Henriksen S A, Buys J, Ruitenberg E J

出版信息

Nord Vet Med. 1975 Apr;27(4):199-202.

PMID:1096076
Abstract

A series of comparative IF tests have been made on serum samples from 9 SPF pigs experimentally infected with Trichinella spiralis (50-500 larvae per animal). The techniques applied included a tube test (antigen: whole Trichinella larvae), and a cryostat test (antigen: sections of Trichinella larvae). With the cryostat test a positive reaction was recorded in 2 animals already on the 6th day and in 5 animals on the 13th day p.i., while with the tube test such reaction was not demonstrated until the 27th day p.i. (2 animals). A potential risk of cross-reaction of unknown origin seems, however, to limit the application of the cryostat test to SPF- pigs.

摘要

对9只经实验感染旋毛虫(每只动物感染50 - 500条幼虫)的无特定病原体(SPF)猪的血清样本进行了一系列比较性免疫荧光(IF)试验。应用的技术包括试管试验(抗原:旋毛虫整条幼虫)和低温恒温器试验(抗原:旋毛虫幼虫切片)。在低温恒温器试验中,在感染后第6天就有2只动物记录到阳性反应,在感染后第13天有5只动物出现阳性反应,而在试管试验中,直到感染后第27天才有2只动物出现阳性反应。然而,来源不明的交叉反应潜在风险似乎限制了低温恒温器试验在SPF猪中的应用。

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1
[Immunofluorescence technique in diagnosis of trichinellosis in swine. II. Comparative investigations with the tube test and the cryostate method (author's transl)].[免疫荧光技术在猪旋毛虫病诊断中的应用。II. 与试管试验和冷冻切片法的比较研究(作者译)]
Nord Vet Med. 1975 Apr;27(4):199-202.
2
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