Macfarlane R D, Hu Z H, Song S, Pittenauer E, Schmid E R, Allmaier G, Metzger J O, Tuszynski W
Texas A&M University, Department of Chemistry, College Station 77843-3255.
Biol Mass Spectrom. 1994 Mar;23(3):117-30. doi: 10.1002/bms.1200230302.
The 252Cf-PDMS method is a 'mature' technique, having survived 20 years of research, development and application. Recent advances in the application of the method have led to the use of the technique in clinical quantitation studies, the development of a microscopic method for measuring chemical homogeneity, in situ applications where serial modifications can be made and studied in a sequential fashion and the utilization of the primary fragmentation patterns to correlate with primary structure. Future directions in research, development and application will focus more attention on the chemistry of the 252Cf-PDMS process and the understanding and control of gas-phase reactions that occur in the ejection plume. Predictions are that the application of the in situ modification technique could lead to a new approach to pharmacokinetic studies as well as cell surface interactions where 252Cf-PDMS is effectively used to expand the capabilities of in vitro studies of biological processes.
252Cf-PDMS方法是一项“成熟”技术，历经20年的研究、开发和应用。该方法应用方面的最新进展已使其用于临床定量研究、开发一种测量化学均匀性的微观方法、可进行系列修饰并以连续方式进行研究的原位应用以及利用初级裂解模式与一级结构相关联。研究、开发和应用的未来方向将更多地关注252Cf-PDMS过程的化学以及对喷射羽流中发生的气相反应的理解和控制。预计原位修饰技术的应用可能会带来一种新的药代动力学研究方法以及细胞表面相互作用研究方法，其中252Cf-PDMS可有效用于扩展生物过程体外研究的能力。
