Kropinski A M, Chadwick J S
Can J Microbiol. 1975 Dec;21(12):2084-8. doi: 10.1139/m75-297.
Rough mutants of two strains of Pseudomonas aeruginosa have been isolated by selection for resistance to virulent lipopolysaccharide-specific phages. The rough mutants fell into two classes on the basis of colonial morphology and agglutination by acriflavine and NaCl. The pigment and exoenzyme-synthesizing properties of these derivatives were identical with those of the parental cultures. The rough strains were 8- to 62-fold less pathogenic for the larvae of the wax moth (Galleria mellonella) than the smooth wild-type cells.
通过选择对毒性脂多糖特异性噬菌体具有抗性，已分离出两株铜绿假单胞菌的粗糙突变体。基于菌落形态以及吖啶黄素和氯化钠的凝集反应，粗糙突变体分为两类。这些衍生物的色素和外酶合成特性与亲代培养物相同。与光滑的野生型细胞相比，粗糙菌株对大蜡螟（Galleria mellonella）幼虫的致病性低8至62倍。
