酿酒酵母的Yak1蛋白激酶通过一种不依赖Sch9蛋白激酶的机制来调节耐热性并抑制生长。
The Yak1 protein kinase of Saccharomyces cerevisiae moderates thermotolerance and inhibits growth by an Sch9 protein kinase-independent mechanism.
作者信息
Hartley A D, Ward M P, Garrett S
机构信息
Section of Cell Growth, Duke University Medical Center, Durham, North Carolina 27710.
出版信息
Genetics. 1994 Feb;136(2):465-74. doi: 10.1093/genetics/136.2.465.
Abstract
The growth defect associated with the loss of yeast A kinase activity can be alleviated by the overexpression or deletion of two other kinases, Sch9 and Yak1, respectively. Using tests of epistasis, we have shown that Sch9 and Yak1 define separate signaling pathways and must, therefore, suppress the A kinase defect by different mechanisms. Nevertheless, the Yak1 kinase appears to regulate cellular processes that are under A kinase control. For example, acquisition of heat resistance is correlated with Yak1 kinase activity, such that YAK1-overexpressing cells are over 200-fold more resistant than isogenic yak1 strains. These results, for the first time, associate a phenotype, other than suppression of the A kinase growth defect, with the loss of Yak1 activity and argue a broader role for the Yak1 kinase in cell growth.
摘要
与酵母A激酶活性丧失相关的生长缺陷可分别通过另外两种激酶Sch9和Yak1的过表达或缺失得到缓解。通过上位性测试，我们已经表明Sch9和Yak1定义了不同的信号通路，因此，它们必定通过不同的机制抑制A激酶缺陷。然而，Yak1激酶似乎调控着受A激酶控制的细胞过程。例如，耐热性的获得与Yak1激酶活性相关，使得过表达YAK1的细胞比同基因的yak1菌株的耐热性高200多倍。这些结果首次将除抑制A激酶生长缺陷之外的一种表型与Yak1活性丧失联系起来，并证明Yak1激酶在细胞生长中具有更广泛的作用。
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